Team:Freiburg bioware/Notebook/His+FluA+Split+FokI pMA

Commented GenBank file

His+FluA+SplitLinker+Foki in pMA Vector

    1  gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa
       r  w  h  f   s  g  k   c  a  r   n  p  y  l   f  i  f   l  n  t

   61  caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
       gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt
       f  k  y  v   s  a  h   e  t  i   t  l  i  n   a  s  i   i  l  k

  121  ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
       ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa
       k  e  e  y   e  y  s   t  f  p   c  r  p  y   s  l  f   c  g  i
               >>........................AmpR..........................>
                  m  s  i  q   h  f  r   v  a  l   i  p  f  f   a  a  f

  181  gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
       cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca
       l  p  s  c   f  c  s   p  r  n   a  g  e  s   k  r  c   -  r  s

       >.............................AmpR..............................>
        c  l  p   v  f  a  h   p  e  t   l  v  k   v  k  d  a   e  d  q

  241  tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa
       v  g  c  t   s  g  l   h  r  t   g  s  q  q   r  -  d   p  -  e
       >.............................AmpR..............................>
        l  g  a   r  v  g  y   i  e  l   d  l  n   s  g  k  i   l  e  s

  301  ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
       aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc
       f  s  p  r   r  t  f   s  n  d   e  h  f  -   s  s  a   m  w  r
       >.............................AmpR..............................>
        f  r  p   e  e  r  f   p  m  m   s  t  f   k  v  l  l   c  g  a

  361  tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
       ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct
       g  i  i  p   y  -  r   r  a  r   a  t  r  s   p  h  t   l  f  s

       >.............................AmpR..............................>
        v  l  s   r  i  d  a   g  q  e   q  l  g   r  r  i  h   y  s  q

  421  atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
       tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt
       e  -  l  g   -  v  l   t  s  h   r  k  a  s   y  g  w   h  d  s
       >.............................AmpR..............................>
        n  d  l   v  e  y  s   p  v  t   e  k  h   l  t  d  g   m  t  v

  481  gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
       ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact
       k  r  i  m   q  c  c   h  n  h   e  -  -  h   c  g  q   l  t  s
       >.............................AmpR..............................>
        r  e  l   c  s  a  a   i  t  m   s  d  n   t  a  a  n   l  l  l

  541  caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
       gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt
       d  n  d  r   r  t  e   g  a  n   r  f  f  a   q  h  g   g  s  c

       >.............................AmpR..............................>
        t  t  i   g  g  p  k   e  l  t   a  f  l   h  n  m  g   d  h  v

  601  ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
       gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt
       n  s  p  -   s  l  g   t  g  a   e  -  s  h   t  k  r   r  a  -
       >.............................AmpR..............................>
        t  r  l   d  r  w  e   p  e  l   n  e  a   i  p  n  d   e  r  d

  661  ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
       ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat
       h  h  d  a   c  s  n   g  n  n   v  a  q  t   i  n  w   r  t  t
       >.............................AmpR..............................>
        t  t  m   p  v  a  m   a  t  t   l  r  k   l  l  t  g   e  l  l

  721  ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
       gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg
       y  s  s  f   p  a  t   i  n  r   l  d  g  g   g  -  s   c  r  t

       >.............................AmpR..............................>
        t  l  a   s  r  q  q   l  i  d   w  m  e   a  d  k  v   a  g  p

  781  ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
       aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg
       t  s  a  l   g  p  s   g  w  l   v  y  c  -   -  i  w   s  r  -
       >.............................AmpR..............................>
        l  l  r   s  a  l  p   a  g  w   f  i  a   d  k  s  g   a  g  e

  841  gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
       caccaagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc
       a  w  f  s   r  y  h   c  s  t   g  a  r  w   -  a  l   p  y  r
       >.............................AmpR..............................>
        r  g  s   r  g  i  i   a  a  l   g  p  d   g  k  p  s   r  i  v

  901  ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
       aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct
       s  y  l  h   d  g  e   s  g  n   y  g  -  t   k  -  t   d  r  -

       >.............................AmpR..............................>
        v  i  y   t  t  g  s   q  a  t   m  d  e   r  n  r  q   i  a  e

  961  taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
       atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa
       d  r  c  l   t  d  -   a  l  v   t  v  r  p   s  l  l   i  y  t
       >............AmpR............>>
        i  g  a   s  l  i  k   h  w  -

 1021  agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
       tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat
       l  d  -  f   k  t  s   f  l  i   -  k  d  l   g  e  d   p  f  -

 1081  atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc
       -  s  h  d   q  n  p   l  t  -   v  f  v  p   l  s  v   r  p  r

 1141  aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
       ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt
       r  k  d  q   r  i  f   l  r  s   f  f  s  a   r  n  l   l  l  a

 1201  caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
       gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa
       n  k  k  t   t  a  t   s  g  g   l  f  a  g   s  r  a   t  n  s

 1261  ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
       aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg
       f  s  e  g   n  w  l   q  q  s   a  d  t  k   y  c  s   s  s  v

 1321  cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
       gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt
       a  v  v  r   p  p  l   q  e  l   c  s  t  a   y  i  p   r  s  a

 1381  tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
       aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt
       n  p  v  t   s  g  c   c  q  w   r  -  v  v   s  y  r   v  g  l

 1441  gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
       ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg
       k  t  i  v   t  g  -   g  a  a   v  g  l  n   g  g  f   v  h  t

 1501  ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
       ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt
       a  q  l  g   a  n  d   l  h  r   t  e  i  p   t  a  -   a  m  r

 1561  gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
       cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt
       k  r  h  a   s  r  r   e  k  g   g  q  v  s   g  k  r   q  g  r

 1621  caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
       gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc
       n  r  r  a   h  e  g   a  s  r   g  k  r  l   v  s  l   -  s  c

 1681  ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
       ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg
       r  v  s  p   p  l  t   -  a  s   i  f  v  m   l  v  r   g  a  e

 1741  tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
       ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac
       p  m  e  k   r  q  q   r  g  l   f  t  v  p   g  l  l   l  a  f

 1801  ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
       gagtaatccg tggggtccga aatgggcttg ctggctcgcg tcgctcagtc actcgctcct
       c  s  l  g   t  p  g   f  t  r   t  t  e  r   s  e  s   v  s  e

 1861  agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
       tcgcctctcg cgggttatgc gttcctttgt cgatactggt acaattacgt cgaccgtgct
       e  a  e  s   a  q  y   a  r  k   q  l  -  p   c  -  c   s  w  h

 1921  caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
       gtccaaaggg ctgacctttc gcccgtcact cgccttccgg gtactccggt caattaattc
       d  r  f  p   d  w  k   a  g  s   e  r  k  a   h  e  a   s  -  l

                        NotI
                     --+------
               EcoRI            XbaI    NgoMIV

              -+-----          -+----   -+----
 1981  aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
       tccatggctt aagcgccggc gaagatctac cggccggtag tagtagtagt agtatggccg
       r  g  t  e   f  a  a   a  s  r   w  p  a  i   i  i  i   i  i  p
                                    >>.............ORF-1...............>
                                      m   a  g  h   h  h  h   h  h  t  g

                                              >>....His Tag.....>>
                                                h   h  h  h   h  h

 2041  gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
       ctgcacatgg tgctgccgcg gacggggctt cacttcgggc acctgttgaa gctgaccagg
       a  t  c  t   t  t  a   p  a  p   k  -  s  p   w  t  t   s  t  g
       >.............................ORF-1.............................>
         d  v  y   h  d  g   a  c  p  e   v  k  p   v  d  n   f  d  w  s

       >>............................FluA..............................>
         d  v  y   h  d  g   a  c  p  e   v  k  p   v  d  n   f  d  w  s

 2101  cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
       gtcatggtgc cgttcaccac cctccaccgg ttcatggggt cggggttgcc gttcataccg
       p  s  t  t   a  s  g   g  r  w   p  s  t  p   a  p  t   a  s  m
       >.............................ORF-1.............................>
         q  y  h   g  k  w   w  e  v  a   k  y  p   s  p  n   g  k  y  g

       >.............................FluA..............................>
         q  y  h   g  k  w   w  e  v  a   k  y  p   s  p  n   g  k  y  g

 2161  aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
       ttcacgccga cccggctcat gtgggggctc ccgttctcgc acttccacag gtctatgctg
       a  s  a  a   g  p  s   t  p  p   r  a  r  a   -  r  c   p  d  t
       >.............................ORF-1.............................>
         k  c  g   w  a  e   y  t  p  e   g  k  s   v  k  v   s  r  y  d

       >.............................FluA..............................>
         k  c  g   w  a  e   y  t  p  e   g  k  s   v  k  v   s  r  y  d

 2221  gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
       cactaggtgc cgtttcttat gaagtacctt ccgtggcgga tggggcaccc gctgtcgttc
       t  -  s  t   a  k  n   t  s  w   k  a  p  p   t  p  w   a  t  a
       >.............................ORF-1.............................>
         v  i  h   g  k  e   y  f  m  e   g  t  a   y  p  v   g  d  s  k

       >.............................FluA..............................>
         v  i  h   g  k  e   y  f  m  e   g  t  a   y  p  v   g  d  s  k

 2281  atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
       tagccgttct agatggtgtc ggcctggcac ccgccgatgt ggtctttctg gcacaagttg
       r  s  a  r   s  t  t   a  g  p   w  a  a  t   p  e  r   p  c  s
       >.............................ORF-1.............................>
         i  g  k   i  y  h   s  r  t  v   g  g  y   t  r  k   t  v  f  n

       >.............................FluA..............................>
         i  g  k   i  y  h   s  r  t  v   g  g  y   t  r  k   t  v  f  n

 2341  gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
       cacgacaggt ggctgttgtt cttgatgtag tagccgatgt cgacggcgat gctgctcctg
       t  c  c  p   p  t  t   r  t  t   s  s  a  t   a  a  a   t  t  r
       >.............................ORF-1.............................>
         v  l  s   t  d  n   k  n  y  i   i  g  y   s  c  r   y  d  e  d

       >.............................FluA..............................>
         v  l  s   t  d  n   k  n  y  i   i  g  y   s  c  r   y  d  e  d

 2401  aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
       ttcttcccgg tgaccctggt gcacacccac gacagggcca ggtaccacga ctggccgctc
       t  r  r  a   t  g  t   t  c  g   c  c  p  g   p  w  c   -  p  a
       >.............................ORF-1.............................>
         k  k  g   h  w  d   h  v  w  v   l  s  r   s  m  v   l  t  g  e

       >.............................FluA..............................>
         k  k  g   h  w  d   h  v  w  v   l  s  r   s  m  v   l  t  g  e

 2461  gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
       cggttctggc ggcacctctt gatggactag ccgtcggggc accacctgtc ggtctttgac
       r  p  r  p   p  w  r   t  t  -   s  a  a  p   w  w  t   a  r  n
       >.............................ORF-1.............................>
         a  k  t   a  v  e   n  y  l  i   g  s  p   v  v  d   s  q  k  l

       >.............................FluA..............................>
         a  k  t   a  v  e   n  y  l  i   g  s  p   v  v  d   s  q  k  l

 2521  gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt
       cacatgtcgc tgaagtcgct ccggcggacg tttcacttgt tgtggccggc tggtcggaca
       w  c  t  a   t  s  a   r  p  p   a  k  -  t   t  p  a   d  q  p
       >.............................ORF-1.............................>
         v  y  s   d  f  s   e  a  a  c   k  v  n   n  t  g   r  p  a  c

       >....................FluA...................>>
         v  y  s   d  f  s   e  a  a  c   k  v  n   n
                                              Split-Linker >>..........>
                                                              r  p  a  c

 2581  aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
       ttctaaggtt tactggactt cgtctttcaa tacttagtgt ggccgtttag acttgacctc
       v  r  f  q   m  t  -   s  r  k   l  -  i  t   p  a  n   l  n  w

       >.............................ORF-1.............................>
         k  i  p   n  d  l   k  q  k  v   m  n  h   t  g  k   s  e  l  e
       >..............Split-Linker.............>>
         k  i  p   n  d  l   k  q  k  v   m  n  h
                                                     >>.'Fok inaktiv'..>

                                                       g  k   s  e  l  e

 2641  gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
       ctctttttta ggctcgacgc ggtgtttgac tttatacacg gagtgctcat atagcttgac
       r  r  k  n   p  s  c   a  t  n   -  n  m  c   l  t  s   i  s  n
       >.............................ORF-1.............................>
         e  k  k   s  e  l   r  h  k  l   k  y  v   p  h  e   y  i  e  l

       >.........................'Fok inaktiv'.........................>
         e  k  k   s  e  l   r  h  k  l   k  y  v   p  h  e   y  i  e  l

 2701  atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
       tagctctagc gggcattatc atgggttctg gcataggacc tttactttca ctacctcaag
       -  s  r  s   p  v  i   v  p  k   t  v  s  w   k  -  k   -  w  s
       >.............................ORF-1.............................>
         i  e  i   a  r  n   s  t  q  d   r  i  l   e  m  k   v  m  e  f

       >.........................'Fok inaktiv'.........................>
         i  e  i   a  r  n   s  t  q  d   r  i  l   e  m  k   v  m  e  f

 2761  ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt
       aagtactttc agataccgat agcaccgttt gtagacccac catcggcatt tggtcgtcca
       s  s  -  k   s  m  a   i  v  a   n  i  w  v   v  a  v   n  q  q
       >.............................ORF-1.............................>
         f  m  k   v  y  g   y  r  g  k   h  l  g   g  s  r   k  p  a  g

       >.........................'Fok inaktiv'.........................>
         f  m  k   v  y  g   y  r  g  k   h  l  g   g  s  r   k  p  a  g

 2821  gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat
       cggtaaatat ggcaaccaag gggctagcta ataccgcaat agcaccggtg ttttcgcata
       v  p  f  i   p  l  v   p  r  s   i  m  a  l   s  w  p   q  k  r
       >.............................ORF-1.............................>
         a  i  y   t  v  g   s  p  i  d   y  g  v   i  v  a   t  k  a  y

       >.........................'Fok inaktiv'.........................>
         a  i  y   t  v  g   s  p  i  d   y  g  v   i  v  a   t  k  a  y

 2881  tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag
       agaccgccaa tattagacgg ctaaccagtc cgactactct accttgcaat acaccttctc
       i  l  a  v   i  i  c   r  l  v   r  l  m  r   w  n  v   m  w  k
       >.............................ORF-1.............................>
         s  g  g   y  n  l   p  i  g  q   a  d  e   m  e  r   y  v  e  e

       >.........................'Fok inaktiv'.........................>
         s  g  g   y  n  l   p  i  g  q   a  d  e   m  e  r   y  v  e  e

 2941  aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt
       ttagtctggg cattgtttgt agacttgggc ttgcttacca cctttcacat aggcagttca
       r  i  r  p   v  t  n   i  -  t   r  t  n  g   g  k  c   i  r  q
       >.............................ORF-1.............................>
         n  q  t   r  n  k   h  l  n  p   n  e  w   w  k  v   y  p  s  s

       >.........................'Fok inaktiv'.........................>
         n  q  t   r  n  k   h  l  n  p   n  e  w   w  k  v   y  p  s  s

 3001  gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag
       cagtggctca agtttaaaga caagcactcg ccggtgaaat ttccgttgat atttcgggtc
       v  s  p  s   s  n  f   c  s  -   a  a  t  l   k  a  t   i  k  p
       >.............................ORF-1.............................>
         v  t  e   f  k  f   l  f  v  s   g  h  f   k  g  n   y  k  a  q

       >.........................'Fok inaktiv'.........................>
         v  t  e   f  k  f   l  f  v  s   g  h  f   k  g  n   y  k  a  q

 3061  ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg
       gactgagcag acttggtata gtggttatcg ttaccccgtc acgactcaca actccttgac
       s  -  l  v   -  t  i   s  p  i   a  m  g  q   c  -  v   l  r  n
       >.............................ORF-1.............................>
         l  t  r   l  n  h   i  t  n  s   n  g  a   v  l  s   v  e  e  l

       >.........................'Fok inaktiv'.........................>
         l  t  r   l  n  h   i  t  n  s   n  g  a   v  l  s   v  e  e  l

 3121  ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa
       gactagccac ctctttacta gtttcgtccg tgggactgag accttcttca agcggcattt
       c  -  s  v   e  k  -   s  k  q   a  p  -  l   w  k  k   f  a  v
       >.............................ORF-1.............................>
         l  i  g   g  e  m   i  k  a  g   t  l  t   l  e  e   v  r  r  k

       >.........................'Fok inaktiv'.........................>
         l  i  g   g  e  m   i  k  a  g   t  l  t   l  e  e   v  r  r  k

                                                             PstI
                                                           ------+
                                             SpeI     NotI

                                            -+----  --+-----
 3181  ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca
       aagttgttac cgctctagtt aaaatggcca attatgatca tcgccggcga cgtctcgagt
       n  s  t  m   a  r  s   i  l  p   v  n  t  s   s  g  r   c  r  a
       >..............ORF-1..............>>
         f  n  n   g  e  i   n  f  t  g   -

       >..........'Fok inaktiv'.........>>
         f  n  n   g  e  i   n  f  t  g

 3241  tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
       accgcgcgga tccggaactg ccggaaggcg gttaagcggg atatcactca gcataatgca
       h  g  a  p   r  p  -   r  p  s   a  n  s  p   y  s  e   s  y  y

 3301  cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
       gcgcgagtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg
       v  a  l  t   g  r  r   f  t  t   s  -  l  g   k  p  w   r  y  p

 3361  ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
       aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt
       t  -  s  p   c  s  t   s  p  f   r  q  l  a   -  -  r   r  g  p

 3421  ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
       ggctttgcgg gaagggttgt caacgcgtcg gacttaccgc ttaccctcgc gggacatcgc
       h  r  n  a   l  p  n   s  c  a   a  -  m  a   n  g  s   a  l  -

 3481  gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
       cggtgagttg ggatagagcc agataagaaa actaaatatt ccctaaaacg gctaaagccg
       r  p  l  n   p  i  s   v  y  s   f  d  l  -   g  i  l   p  i  s

 3541  ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
       gataaccaat tttttactcg actaaattgt ttttaaattg cgcttaaaat tgttttataa
       a  y  w  l   k  n  e   l  i  -   q  k  f  n   a  n  f   n  k  i

 3601  aacgcttaca atttag
       ttgcgaatgt taaatc
       l  t  l  t   i  -

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