Team:Freiburg bioware/cloning

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   <li><a href="https://2009.igem.org/Team:Freiburg_bioware"
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  class="r"></span><span class="t">Home</span></a></li>
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   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project"><span class="l"></span><span
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Project"><span class="l"></span><span
  class="r"></span><span class="t">The
  class="r"></span><span class="t">The
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Project</span></a></li>
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Project</span></a><ul>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
 +
      </li>
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      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
 +
    </ul></li>
   <li><a
   <li><a
  href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice"><span
  href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice"><span
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  class="r"></span><span class="t">Notebook</span></a></li>
  class="r"></span><span class="t">Notebook</span></a></li>
   <li><a
   <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/cloning1" class="active"><span
 +
class="l"></span><span class="r"></span><span
 +
class="t">Parts</span></a><ul>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic
 +
Parts</a></li>
 +
      <li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite
 +
Parts</a></li>
 +
    </ul></li>
 +
<li><a
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
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<div class="art-PostMetadataHeader">
<div class="art-PostMetadataHeader">
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<h2 class="art-PostHeaderIcon-wrapper"> &nbsp;Parts<span class="art-PostHeader"></span> </h2>
+
 
 +
<p></div><a name="1"></a>
 +
<h3>Index</h3><br>
 +
 
 +
 
 +
<table border="0">
 +
<tr><td>
 +
<p><font size="2">
 +
*<a href="#2"> His+FluA+Split+Foki_pMA
 +
</a><br>
 +
*<a href="#3"> His+Dig+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#4">
 +
DsbA+His+Dig+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#36">
 +
DsbA+Strep+Dig+Split+FokA_pEX
 +
</a><br>
 +
*<a href="#5">
 +
Strep+FluA+ShortLinker+FokI_pMA
 +
</a><br>
 +
*<a href="#6">
 +
His+FluA+middellinker+FokA_pMA
 +
</a><br>
 +
*<a href="#7">
 +
His+FluA+middellinker+Foki_pMA
 +
</a><br>
 +
*<a href="#8">
 +
His+FluA+shortlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#9">
 +
His+FluA+shortlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#10">
 +
His+Dig+longlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#11">
 +
His+Dig+longlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#12">
 +
Strep+FluA+Split+Foki_pMA
 +
</a><br>
 +
*<a href="#13">
 +
His+Dig+middellinker+FokA_pMA
 +
</a><br>
 +
*<a href="#14">
 +
His+Dig+middellinker+Foki_pMA
 +
</a><br>
 +
*<a href="#15">
 +
His+Dig+shortlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#16">
 +
His+Dig+shortlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#17">
 +
His+Dig+Split+Foki_pMA
 +
</a><br>
 +
</p></font></p>
 +
</td>
 +
 
 +
<td>
 +
<p><font size="2">
 +
*<a href="#18">
 +
His+FluA+longlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#19">
 +
His+FluA+longlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#20">
 +
His+FluA+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#21">
 +
Strep+Dig+longlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#22">
 +
Strep+Dig+longlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#23">
 +
Strep+Dig+middellinker+FokA_pMA
 +
</a><br>
 +
*<a href="#24">
 +
Strep+Dig+middellinker+Foki_pMA
 +
</a><br>
 +
*<a href="#25">
 +
Strep+FluA+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#26">
 +
Strep+FluA+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#27">
 +
Strep+Dig+shortlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#28">
 +
Strep+Dig+shortlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#29">
 +
Strep+Dig+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#30">
 +
Strep+Dig+Split+Foki_pMA
 +
</a><br>
 +
*<a href="#31">
 +
Strep+FluA+longlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#32">
 +
Strep+FluA+longlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#33">
 +
Strep+FluA+middellinker+FokA_pMA
 +
</a><br>
 +
*<a href="#34">
 +
Strep+FluA+middellinker+Foki_pMA
 +
</a><br>
 +
</p></font></p>
 +
</td>
 +
 
 +
 
 +
<td>
 +
 
 +
<p><font size="2">
 +
*<a href="#35">
 +
Strep+FluA+shortlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#37">
 +
FokA+YFP(Venus)_pEX
 +
</a><br>
 +
*<a href="#38">
 +
Longlinker+FokA_pMA
 +
</a><br>
 +
*<a href="#39">
 +
Longlinker+Foki_pMA
 +
</a><br>
 +
*<a href="#40">
 +
middelLinker+FokA_pMA
 +
</a><br>
 +
*<a href="#41">
 +
ShortLinker26+FokA_pMA
 +
</a><br>
 +
*<a href="#42">
 +
Strep+Dig_pMA
 +
</a><br>
 +
*<a href="#43">
 +
Strep+FluA_pMA
 +
</a><br>
 +
*<a href="#44">
 +
Split+Foki_pMA
 +
</a><br>
 +
*<a href="#45">
 +
His+FluA_pMA
 +
</a><br>
 +
*<a href="#46">
 +
Fos+Split+FokA_pMA
 +
</a><br>
 +
*<a href="#47">
 +
His+Dig+Split+FokA_pJS419
 +
</a><br>
 +
<br><br><br><br><br>
 +
</p></font></p>
 +
</td></tr></table>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;Parts<span class="art-PostHeader"></span> </h2>
</div>
</div>
 +
</div><a name="2"></a>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
-
</div>
 
<div class="art-PostContent">
<div class="art-PostContent">
-
<div style="text-align: center;">
+
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
<table border="5">
<table border="5">
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<img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
<img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
<br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p>
<br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243010">link to part registry</a>
</td>
</td>
<td align="left">
<td align="left">
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<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
<li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
<li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
-
 
+
<br>
-
 
+
<br>
 +
<br>
 +
</div><a name="3"></a>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
-
</div>
+
 
<div class="art-PostContent">
<div class="art-PostContent">
-
<div style="text-align: center;">
+
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
<table border="5">
<table border="5">
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<td align="center">
<td align="center">
<img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
<img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
-
<br><p align="center">His+Dig+Split+FokA_pMA in pMA Vector</p>
+
<br><p align="center">His+Dig+Split+FokA in pMA Vector</p>
</td>
</td>
<td align="left">
<td align="left">
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<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt</a></li>
+
<li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt">His+Dig+Split+FokA_pMA.txt</a></li>
-
<div style="text-align: left;">
+
<br>
 +
<br>
 +
<br>
 +
</div><a name="4"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA+His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/4/4f/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.JPG" name="DsbA+His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">DsbA+His+Dig+Split+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243021">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
 +
ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG
 +
ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG
 +
TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA
 +
GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA
 +
AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC
 +
ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA
 +
CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG
 +
ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG
 +
AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC
 +
CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG
 +
TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA
 +
GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC
 +
AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG
 +
GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA
 +
TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC
 +
CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT
 +
ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC
 +
ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA
 +
AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC
 +
AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC
 +
GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT
 +
CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA
 +
ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG
 +
ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG
 +
CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
 +
YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
 +
GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
 +
SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE
 +
KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA
 +
IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV
 +
TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF
 +
NNGEINFTG
 +
<br><br></p></td></table>
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/4d/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.txt">DsbA+His+Dig+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
</div><a name="36"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA+Strep+Dig+Split+FokA_pEX<span class="art-PostHeader"></span> </h3>
</div>
</div>
-
<br />
+
<div class="art-PostContent">
-
<div style="text-align: center;">
+
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/8/82/Freiburg09_DsbA%2BStrep%2BDig%2BSplit%2BFokA_pEX.jpg/600px-Freiburg09_DsbA%2BStrep%2BDig%2BSplit%2BFokA_pEX.jpg" name="DsbA+Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">DsbA+Strep+Dig+Split+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243022">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
 +
ATCGGCGACCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGT
 +
ACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGG
 +
TCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACAT
 +
CACCAAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGA
 +
GCGTGAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGC
 +
GAGGGCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCA
 +
CAGCTACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGT
 +
CCACCGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAG
 +
GACAAGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGT
 +
GCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCC
 +
CCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCC
 +
TGCAAAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGG
 +
CCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATC
 +
ACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAA
 +
CTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAA
 +
TAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGA
 +
AAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGAT
 +
GGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGA
 +
TACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATG
 +
AGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAAC
 +
CCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATT
 +
CCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCC
 +
GTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAG
 +
CTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGA
 +
AGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MKKIWLALAGLVLAFSASATGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQV
 +
AAYPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYT
 +
IGGVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYL
 +
IGSPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSEL
 +
EEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPD
 +
GAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPS
 +
SVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRR
 +
KFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/a/aa/Freiburg09_DsbA%2BStrep%2BDig%2BSplit%2BFokA_pEX.txt">DsbA+Strep+Dig+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</div><a name="5"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+ShortLinker+FokI_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
-
<br />
+
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/0/03/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.jpg" name="Strep+FluA+ShortLinker+FokI in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+ShortLinker+FokI in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCC
 +
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 +
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 +
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 +
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 +
ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA
 +
AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA
 +
ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA
 +
AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG
 +
GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT
 +
CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC
 +
TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG
 +
ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC
 +
AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA
 +
ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG
 +
GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC
 +
AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA
 +
TCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 +
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 +
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
 +
KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ
 +
TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI
 +
GGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/7e/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.txt">Strep+FluA+ShortLinker+FokI_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="6"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
-
<div class="cleared"></div>
+
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 +
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 +
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 +
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 +
CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC
 +
GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA
 +
ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC
 +
CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC
 +
GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG
 +
GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT
 +
GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA
 +
AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG
 +
AGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 +
MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN
 +
QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL
 +
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="7"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p><br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243014">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 +
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 +
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 +
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 +
CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
 +
GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
 +
ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
 +
CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
 +
GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
 +
GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
 +
GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
 +
AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
 +
AGATCAATTTTACCGGTTAA
 +
 +
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 +
MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
 +
QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
 +
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
</div><a name="8"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
 +
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
 +
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
 +
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
 +
CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
 +
CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
 +
GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
 +
AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
 +
AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
 +
CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
 +
TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
 +
GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
 +
CCGGTTAA
 +
 +
 +
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
 +
GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
 +
KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
 +
MIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
</div><a name="9"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
</div>
</div>
-
<div class="art-Post">
+
<div class="art-PostContent">
-
<div class="art-Post-tl"></div>
+
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
-
<div class="art-Post-tr"></div>
+
 
-
<div class="art-Post-bl"></div>
+
 
-
<div class="art-Post-br"></div>
+
<table border="5">
-
<div class="art-Post-tc"></div>
+
<tr>
-
<div class="art-Post-bc"></div>
+
<td align="center">
-
<div class="art-Post-cl"></div>
+
<img src="https://static.igem.org/mediawiki/2009/thumb/b/bc/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg" name="His+FluA+shortlinker+Foki in pMA Vector" width="480" height="480" />
-
<div class="art-Post-cr"></div>
+
<br><p align="center">His+FluA+shortlinker+Foki in pMA Vector</p><br>
-
<div class="art-Post-cc"></div>
+
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243013">link to part registry</a>
-
<div class="art-Post-body">
+
</td>
-
<div class="art-Post-inner">
+
<td align="left">
-
<div class="art-PostMetadataHeader">
+
nucleotid sequence of ORF-1:<br>
-
<h2 class="art-PostHeaderIcon-wrapper"> <img
+
 
-
src="https://static.igem.org/mediawiki/2009/c/cf/Freiburg09_PostHeaderIcon_2.png" alt="PostHeaderIcon"
+
ATGGCCGGC
-
height="25" width="26" /> Blank<span
+
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
-
class="art-PostHeader"></span> </h2>
+
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
 +
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
 +
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
 +
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
 +
CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
 +
CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG
 +
GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC
 +
AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC
 +
CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG
 +
CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG
 +
AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT
 +
GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA
 +
CCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
 +
GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN
 +
KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE
 +
MIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/7d/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.txt">His+FluA+shortlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="10"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="His+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+longlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 +
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 +
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 +
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 +
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 +
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 +
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 +
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 +
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 +
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 +
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 +
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 +
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 +
TTTACCGGTTAA
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 +
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 +
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 +
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/0/07/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.txt">His+Dig+longlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="11"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/6/64/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG/600px-Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG" name="His+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+longlinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 +
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 +
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 +
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 +
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 +
TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 +
ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG
 +
ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG
 +
TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG
 +
TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT
 +
AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT
 +
GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA
 +
CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT
 +
TTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 +
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP
 +
IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI
 +
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/e/ef/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.txt">His+Dig+longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="12"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCC
 +
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 +
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 +
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 +
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 +
ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
 +
GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
 +
GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
 +
TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
 +
TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
 +
TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
 +
TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
 +
CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
 +
ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
 +
AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
 +
CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
 +
TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
 +
CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
 +
GGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 +
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 +
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
 +
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
 +
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
 +
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="13"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/c/c7/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="His+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 +
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 +
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 +
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 +
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 +
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 +
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
 +
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
 +
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
 +
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
 +
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
 +
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
 +
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 +
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
 +
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
 +
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.txt">His+Dig+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="14"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/3/3b/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="His+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+middellinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 +
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 +
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 +
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 +
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 +
AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 +
TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT
 +
GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT
 +
GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA
 +
AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG
 +
ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA
 +
GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG
 +
AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 +
ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA
 +
DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN
 +
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.txt">His+Dig+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
</div><a name="15"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="His+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+shortlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
 +
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
 +
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
 +
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
 +
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT
 +
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG
 +
CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG
 +
CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA
 +
ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG
 +
TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC
 +
CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT
 +
TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC
 +
GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
 +
KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ
 +
RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL
 +
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.txt">His+Dig+shortlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="16"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/1/15/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg" name="His+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+shortlinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 +
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 +
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 +
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 +
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
 +
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
 +
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
 +
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
 +
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT
 +
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG
 +
CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA
 +
CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA
 +
ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG
 +
TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC
 +
CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT
 +
CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC
 +
GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 +
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 +
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
 +
KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME
 +
RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL
 +
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/b/b5/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.txt">His+Dig+shortlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="17"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d7/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg" name="His+Dig+Split+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+Split+Foki in pMA Vector</p><br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243018">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 +
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 +
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 +
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 +
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
 +
TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
 +
ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
 +
TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
 +
GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
 +
CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT
 +
ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG
 +
TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG
 +
TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT
 +
GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG
 +
AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA
 +
TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG
 +
GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT
 +
AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 +
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 +
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
 +
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA
 +
YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
 +
QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/1/11/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.txt">His+Dig+Split+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="18"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/9f/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="His+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+longlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
 +
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
 +
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
 +
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
 +
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC
 +
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA
 +
GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT
 +
GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG
 +
GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG
 +
GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA
 +
ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG
 +
TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT
 +
TCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
 +
MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
 +
VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV
 +
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/b/be/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.txt">His+FluA+longlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="19"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/6/6c/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="His+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+longlinker+Foki in pMA Vector</p><br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243016">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGC
 +
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 +
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 +
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 +
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 +
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 +
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 +
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 +
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 +
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 +
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 +
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 +
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
 +
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
 +
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
 +
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
 +
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC
 +
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT
 +
CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT
 +
GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG
 +
GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG
 +
GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC
 +
ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG
 +
AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT
 +
TCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 +
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 +
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 +
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
 +
MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY
 +
VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV
 +
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/77/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.txt">His+FluA+longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</div><a name="20"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/e/ef/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg" name="His+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA+Split+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCC
 +
GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
 +
CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
 +
AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
 +
TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG
 +
ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC
 +
CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
 +
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA
 +
CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT
 +
GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC
 +
AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA
 +
GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA
 +
CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG
 +
AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 +
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 +
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 +
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 +
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 +
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
 +
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
 +
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
 +
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
 +
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
 +
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
 +
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
 +
KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
 +
GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
 +
VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 +
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
 +
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
 +
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/5/53/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.txt">His+FluA+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="21"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/8/8b/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="Strep+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+longlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCC
 +
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 +
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 +
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 +
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 +
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
 +
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
 +
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
 +
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
 +
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
 +
TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC
 +
CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT
 +
AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA
 +
CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC
 +
CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA
 +
GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA
 +
TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA
 +
AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG
 +
GAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 +
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 +
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
 +
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY
 +
NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
 +
NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.txt">Strep+Dig+longlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
</div><a name="22"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/6/65/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg" name="Strep+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+longlinker+Foki in pMA Vector <br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243011">link to part registry</a></li>
 +
</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCC
 +
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 +
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 +
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 +
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 +
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
 +
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
 +
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
 +
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
 +
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
 +
TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC
 +
CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT
 +
AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA
 +
TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC
 +
CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA
 +
GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA
 +
TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA
 +
AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC
 +
GAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 +
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 +
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
 +
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY
 +
NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
 +
NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/4c/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.txt">Strep+Dig+longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="23"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 +
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 +
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 +
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 +
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 +
TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
 +
ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
 +
CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
 +
TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 +
GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
 +
TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
 +
CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
 +
GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
 +
AATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
 +
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
 +
PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
 +
KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="24"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/7/79/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+middellinker+Foki in pMA Vector<br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243012">link to part registry</a>
 +
</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 +
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 +
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 +
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 +
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 +
TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG
 +
ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG
 +
CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC
 +
CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 +
GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC
 +
TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC
 +
AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG
 +
GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC
 +
AATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
 +
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL
 +
PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
 +
ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="25"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
 +
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 +
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 +
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 +
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 +
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 +
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 +
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 +
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 +
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 +
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 +
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 +
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 +
TTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 +
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 +
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 +
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="26"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
 +
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 +
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 +
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 +
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 +
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 +
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 +
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 +
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 +
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 +
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 +
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 +
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 +
TTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 +
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 +
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 +
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.txt">Strep+FluA+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="27"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fb/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+shortlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 +
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
 +
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
 +
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
 +
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
 +
ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
 +
TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG
 +
GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA
 +
CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT
 +
TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG
 +
CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT
 +
AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC
 +
TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT
 +
TAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 +
RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ
 +
ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS
 +
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/45/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.txt">Strep+Dig+shortlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="28"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/4/4a/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg" name="Strep+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+shortlinker+Foki in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 +
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
 +
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
 +
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
 +
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
 +
ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
 +
TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG
 +
GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA
 +
TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT
 +
TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG
 +
CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT
 +
TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC
 +
TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT
 +
TAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 +
RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ
 +
ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS
 +
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/0/0f/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.txt">Strep+Dig+shortlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="29"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/f/ff/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg" name="Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+Split+FokA in pMA Vector</p><br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243017">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
 +
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
 +
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
 +
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
 +
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
 +
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG
 +
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC
 +
AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT
 +
GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA
 +
ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG
 +
TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT
 +
GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC
 +
TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT
 +
CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 +
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT
 +
KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 +
KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/95/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.txt">Strep+Dig+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="30"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fe/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg" name="Strep+Dig+Split+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+Split+Foki in pMA Vector</p><br>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243015">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
 +
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
 +
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
 +
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
 +
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
 +
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG
 +
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA
 +
AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT
 +
GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA
 +
ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG
 +
TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT
 +
GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC
 +
TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT
 +
CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 +
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT
 +
KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 +
KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/6/63/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.txt">Strep+Dig+Split+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="31"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/2/22/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="Strep+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+longlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 +
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 +
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 +
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 +
ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC
 +
GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA
 +
ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA
 +
CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC
 +
TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC
 +
CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA
 +
AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG
 +
CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA
 +
CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT
 +
TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG
 +
AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT
 +
GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
 +
GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 +
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 +
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE
 +
MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM
 +
QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV
 +
LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/d/d4/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.txt">Strep+FluA+longlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="32"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part::BBa_K243023">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
 +
GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
 +
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
 +
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
 +
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
 +
AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
 +
CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
 +
GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
 +
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
 +
TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
 +
CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
 +
ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
 +
AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
 +
LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
 +
EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
 +
AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="33"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243024">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 +
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 +
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 +
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 +
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
 +
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
 +
ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
 +
TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
 +
GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
 +
GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
 +
AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
 +
CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
 +
AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 +
VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
 +
YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
 +
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="34"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243025">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 +
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 +
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 +
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 +
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
 +
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
 +
ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
 +
TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
 +
GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
 +
GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
 +
ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
 +
TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
 +
AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 +
VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
 +
YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
 +
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</div><a name="35"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA+shortlinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG
 +
GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA
 +
TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG
 +
AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA
 +
CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC
 +
CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT
 +
ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG
 +
AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA
 +
TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA
 +
AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC
 +
AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT
 +
CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG
 +
GGGAGATCAATTTTACCGGTTAA
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF
 +
FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE
 +
NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL
 +
LIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/49/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.txt">Strep+FluA+shortlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="37"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;FokA+YFP(Venus)_pEX<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_pEX_FokA%2BYFP%28Venus%29.jpg/600px-Freiburg09_pEX_FokA%2BYFP%28Venus%29.jpg" name="FokA+YFP(Venus) in pEX Vector" width="480" height="480" />
 +
<br><p align="center">FokA+YFP(Venus) in pEX Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243032">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
 +
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
 +
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
 +
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA
 +
TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG
 +
ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT
 +
GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA
 +
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT
 +
TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC
 +
CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA
 +
GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG
 +
AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGCATGAGC
 +
AAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGGTGGAACTGGA
 +
TGGTGATGTGAACGGCCATAAATTTAGCGTGAGCGGCGAAGGCGAAGGTG
 +
ATGCGACCTATGGCAAACTGACCCTGAAACTGATTTGCACCACCGGCAAA
 +
CTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCTGGGCTATGGCCTGCA
 +
ATGCTTTGCGCGTTATCCGGATCATATGAAACAGCACGATTTCTTTAAAA
 +
GCGCCATGCCGGAAGGCTATGTGCAGGAACGCACCATCTTTTTTAAAGAT
 +
GATGGCAACTATAAAACCCGTGCGGAAGTGAAATTTGAAGGCGATACCCT
 +
GGTGAACCGTATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGCAACA
 +
TTCTGGGCCATAAACTGGAATATAACTACAACAGCCATAACGTGTATATC
 +
ACCGCGGATAAACAGAAAAACGGCATCAAAGCGAACTTTAAAATCCGCCA
 +
CAACATTGAAGATGGCGGCGTGCAGCTGGCCGATCATTATCAGCAGAACA
 +
CCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGATAACCATTATCTGAGC
 +
TACCAGAGCAAACTGAGCAAAGATCCGAACGAAAAACGTGACCATATGGT
 +
GCTGCTGGAATTTGTGACCGCGGCCGGTATTACCCATGGCATGGATGAAC
 +
TGTATAAAACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL
 +
GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE
 +
WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL
 +
TLEEVRRKFNNGEINFTGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
 +
TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
 +
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
 +
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
 +
FVTAAGITHGMDELYKTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/c/c9/Freiburg09_pEX_FokA%2BYFP%28Venus%29.txt">FokA+YFP(Venus)_pEX.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="38"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/5/5d/Freiburg09_LongLinker%2BFokA_pMA.jpg/600px-Freiburg09_LongLinker%2BFokA_pMA.jpg" name="Longlinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Longlinker+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243026">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGG
 +
CAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAAT
 +
ATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACC
 +
CAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTA
 +
TGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCA
 +
TTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAA
 +
GCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCA
 +
GCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACG
 +
AATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTC
 +
GTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAA
 +
CCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGA
 +
TTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGC
 +
CGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
 +
EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYV
 +
KENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVE
 +
ELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/c/cc/Freiburg09_LongLinker%2BFokA_pMA.txt">Longlinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="39"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_LongLinker%2BFokI_pMA.jpg/600px-Freiburg09_LongLinker%2BFokI_pMA.jpg" name="Longlinker+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Longlinker+Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243009">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGG
 +
CAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAAT
 +
ATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACC
 +
CAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTA
 +
TGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCA
 +
TTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAA
 +
GCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGA
 +
ACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACG
 +
AATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTC
 +
GTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAA
 +
CCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGA
 +
TCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGC
 +
CGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
 +
EFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYV
 +
EENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVE
 +
ELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/3/32/Freiburg09_LongLinker%2BFokI_pMA.txt">Longlinker+Foki_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="40"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;middelLinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_Middellinker38%2BFokA_pMA.jpg/600px-Freiburg09_Middellinker38%2BFokA_pMA.jpg" name="middelLinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">middelLinker+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243008">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACT
 +
GGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACG
 +
AGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATC
 +
CTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGG
 +
CAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTG
 +
GTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGG
 +
GGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAA
 +
AGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAG
 +
TGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCAT
 +
TTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAA
 +
TAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAA
 +
TGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAAC
 +
AATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
 +
KVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQ
 +
TRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLI
 +
GGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/8/8b/Freiburg09_Middellinker38%2BFokA_pMA.txt">middelLinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="41"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;ShortLinker26+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d2/Freiburg09_Shortlinker26%2BFokA_pMA.jpg/600px-Freiburg09_Shortlinker26%2BFokA_pMA.jpg" name="ShortLinker26+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">ShortLinker26+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243007">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 +
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 +
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 +
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 +
TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
 +
ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
 +
CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
 +
TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 +
GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
 +
TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
 +
CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
 +
GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
 +
AATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYG
 +
YRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNK
 +
HINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEM
 +
IKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/4/43/Freiburg09_Shortlinker26%2BFokA_pMA.txt">ShortLinker26+FokA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="42"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Dig%2BStrep_pMA.jpg/600px-Freiburg09_Dig%2BStrep_pMA.jpg" name="Strep+Dig in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243020">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/8/88/Freiburg09_Strep%2Bdig_pMA.txt">Strep+Dig.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="43"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/1/17/Freiburg09_Strep%2BFluA_pMA.jpg/600px-Freiburg09_Strep%2BFluA_pMA.jpg" name="Strep+FluA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+FluA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_S04299">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 +
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 +
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 +
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 +
AAGTGAACAACACCGGTTAA
 +
 
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 +
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 +
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/d/db/Freiburg09_Strep%2BFluA_pMA.txt">Strep+FluA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
</div><a name="44"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/5/52/Freiburg09_Split%2Bfoki_pMA.jpg/600px-Freiburg09_Split%2Bfoki_pMA.jpg" name="Split+Foki in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Split+Foki in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243019">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT
 +
TATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGC
 +
GCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATC
 +
GCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTT
 +
CTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTA
 +
AACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTT
 +
ATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCA
 +
GGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAAC
 +
ATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAG
 +
TTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCA
 +
GCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTG
 +
TTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACT
 +
CTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGG
 +
TTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRIL
 +
EMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADE
 +
MERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGA
 +
VLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/e/e7/Freiburg09_Split%2Bfoki_pMA.txt">Split+Foki.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="45"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/8/8d/Freiburg09_His%2BFluA_pMA.jpg/600px-Freiburg09_His%2BFluA_pMA.jpg" name="His+FluA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">His+FluA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_S04282">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 +
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 +
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 +
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 +
ACAACACCGGTTAA
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 +
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 +
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNTG
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/b/bf/Freiburg09_His%2BFluA_pMA.txt">His+FluA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="46"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Fos+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/c/c3/Freiburg09_Fos%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Fos%2BSplit%2BFokA_pMA.jpg" name="Fos+Split+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Fos+Split+FokA in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243028">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG
 +
TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC
 +
GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC
 +
GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA
 +
AAAACTGGAGTTCATCCTGGCAGCAACCGGCCGACCAGCCTGTAAGATTC
 +
CAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAAATCTGAACTG
 +
GAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGA
 +
GTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCC
 +
TGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGC
 +
AAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGG
 +
TTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGG
 +
GTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAA
 +
GAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGT
 +
GTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATT
 +
TTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAAT
 +
AGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAAT
 +
GATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACA
 +
ATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA
 +
NLLKEKEKLEFILAATGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
 +
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
 +
YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
 +
QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/f/fe/Freiburg09_Fos%2BSplit%2BFokA_pMA.txt">Fos+Split+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
</div><a name="47"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+FokA_pJS419<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 
 +
 
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/6/63/Freiburg09_His%2BDig%2BSplit%2BFokA_pJS419.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokA_pJS419.jpg" name="His+Dig+Split+FokA in pJS419 Vector" width="480" height="480" />
 +
<br><p align="center">His+Dig+Split+FokA in pJS419 Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243036">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 
 +
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 +
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 +
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 +
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 +
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 +
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 +
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 +
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 +
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 +
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 +
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 +
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
 +
TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
 +
ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
 +
TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
 +
GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
 +
CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT
 +
ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC
 +
TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG
 +
TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT
 +
GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG
 +
AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA
 +
TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG
 +
GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT
 +
AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 +
 
 +
 
 +
 
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 +
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 +
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 +
CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
 +
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
 +
YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
 +
QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 
 +
 
 +
 
 +
<br><br></p></td></table>
 +
 
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/75/Freiburg09_His%2BDig%2BSplit%2BFokA_pJS419.txt">His+Dig+Split+FokA_pJS419.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
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Latest revision as of 00:14, 22 October 2009

FREiGEM

Index


* His+FluA+Split+Foki_pMA
* His+Dig+Split+FokA_pMA
* DsbA+His+Dig+Split+FokA_pMA
* DsbA+Strep+Dig+Split+FokA_pEX
* Strep+FluA+ShortLinker+FokI_pMA
* His+FluA+middellinker+FokA_pMA
* His+FluA+middellinker+Foki_pMA
* His+FluA+shortlinker+FokA_pMA
* His+FluA+shortlinker+Foki_pMA
* His+Dig+longlinker+FokA_pMA
* His+Dig+longlinker+Foki_pMA
* Strep+FluA+Split+Foki_pMA
* His+Dig+middellinker+FokA_pMA
* His+Dig+middellinker+Foki_pMA
* His+Dig+shortlinker+FokA_pMA
* His+Dig+shortlinker+Foki_pMA
* His+Dig+Split+Foki_pMA

* His+FluA+longlinker+FokA_pMA
* His+FluA+longlinker+Foki_pMA
* His+FluA+Split+FokA_pMA
* Strep+Dig+longlinker+FokA_pMA
* Strep+Dig+longlinker+Foki_pMA
* Strep+Dig+middellinker+FokA_pMA
* Strep+Dig+middellinker+Foki_pMA
* Strep+FluA+Split+FokA_pMA
* Strep+FluA+Split+FokA_pMA
* Strep+Dig+shortlinker+FokA_pMA
* Strep+Dig+shortlinker+Foki_pMA
* Strep+Dig+Split+FokA_pMA
* Strep+Dig+Split+Foki_pMA
* Strep+FluA+longlinker+FokA_pMA
* Strep+FluA+longlinker+Foki_pMA
* Strep+FluA+middellinker+FokA_pMA
* Strep+FluA+middellinker+Foki_pMA

* Strep+FluA+shortlinker+FokA_pMA
* FokA+YFP(Venus)_pEX
* Longlinker+FokA_pMA
* Longlinker+Foki_pMA
* middelLinker+FokA_pMA
* ShortLinker26+FokA_pMA
* Strep+Dig_pMA
* Strep+FluA_pMA
* Split+Foki_pMA
* His+FluA_pMA
* Fos+Split+FokA_pMA
* His+Dig+Split+FokA_pJS419





 Parts

 His+FluA+Split+Foki_pMA

back to index

His+FluA+SplitLinker+Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
    EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
    IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
    CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
    DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
    QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
    SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA

    back to index

    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA

    back to index

    DsbA+His+Dig+Split+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  DsbA+Strep+Dig+Split+FokA_pEX

    back to index

    DsbA+Strep+Dig+Split+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGT ACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGG TCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACAT CACCAAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGA GCGTGAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGC GAGGGCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCA CAGCTACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGT CCACCGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAG GACAAGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGT GCTGACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCC CCGTGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCC TGCAAAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGG CCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATC ACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAA CTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAA TAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGA AAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGAT GGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGA TACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATG AGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAAC CCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATT CCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCC GTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAG CTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGA AGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQV AAYPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYT IGGVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYL IGSPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSEL EEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPD GAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPS SVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRR KFNNGEINFTG

    Commented GenBank file

  • DsbA+Strep+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA

    back to index

    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA

    back to index

    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA

    back to index

    His+FluA+middellinker+Foki in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA

    back to index

    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt



    •  His+FluA+shortlinker+Foki_pMA

    back to index

    His+FluA+shortlinker+Foki in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+Foki_pMA.txt



    •  His+Dig+longlinker+FokA_pMA

    back to index

    His+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+FokA_pMA.txt



    •  His+Dig+longlinker+Foki_pMA

    back to index

    His+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+Foki_pMA.txt



    •  Strep+FluA+Split+Foki_pMA

    back to index

    Strep+FluA+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+Foki_pMA.txt



    •  His+Dig+middellinker+FokA_pMA

    back to index

    His+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+FokA_pMA.txt



    •  His+Dig+middellinker+Foki_pMA

    back to index

    His+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+Foki_pMA.txt



    •  His+Dig+shortlinker+FokA_pMA

    back to index

    His+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+FokA_pMA.txt



    •  His+Dig+shortlinker+Foki_pMA

    back to index

    His+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+Foki_pMA.txt



    •  His+Dig+Split+Foki_pMA

    back to index

    His+Dig+Split+Foki in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+Foki_pMA.txt



    •  His+FluA+longlinker+FokA_pMA

    back to index

    His+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT TCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+FokA_pMA.txt



    •  His+FluA+longlinker+Foki_pMA

    back to index

    His+FluA+longlinker+Foki in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGC CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT TCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+Foki_pMA.txt



    •  His+FluA+Split+FokA_pMA

    back to index

    His+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+longlinker+FokA_pMA

    back to index

    Strep+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+FokA_pMA.txt



    •  Strep+Dig+longlinker+Foki_pMA

    back to index

    Strep+Dig+longlinker+Foki in pMA Vector

  • link to part registry

    		•  nucleotid sequence of ORF-1:
    ATGGCC GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+Foki_pMA.txt



    •  Strep+Dig+middellinker+FokA_pMA

    back to index

    Strep+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+FokA_pMA.txt



    •  Strep+Dig+middellinker+Foki_pMA

    back to index

    Strep+Dig+middellinker+Foki in pMA Vector

  • link to part registry

    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+Foki_pMA.txt



    •  Strep+FluA+Split+FokA_pMA

    back to index

    Strep+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+shortlinker+FokA_pMA

    back to index

    Strep+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+FokA_pMA.txt



    •  Strep+Dig+shortlinker+Foki_pMA

    back to index

    Strep+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT TAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+shortlinker+Foki_pMA.txt



    •  Strep+Dig+Split+FokA_pMA

    back to index

    Strep+Dig+Split+FokA in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+FokA_pMA.txt



    •  Strep+Dig+Split+Foki_pMA

    back to index

    Strep+Dig+Split+Foki in pMA Vector


  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+Split+Foki_pMA.txt



    •  Strep+FluA+longlinker+FokA_pMA

    back to index

    Strep+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+FokA_pMA.txt



    •  Strep+FluA+longlinker+Foki_pMA

    back to index

    Strep+FluA+longlinker+Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+longlinker+Foki_pMA.txt



    •  Strep+FluA+middellinker+FokA_pMA

    back to index

    Strep+FluA+middellinker+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA AATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+FokA_pMA.txt



    •  Strep+FluA+middellinker+Foki_pMA

    back to index

    Strep+FluA+middellinker+Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA AATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+middellinker+Foki_pMA.txt



    •  Strep+FluA+shortlinker+FokA_pMA

    back to index

    Strep+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG GGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL LIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+shortlinker+FokA_pMA.txt



    •  FokA+YFP(Venus)_pEX

    back to index

    FokA+YFP(Venus) in pEX Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGCATGAGC AAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGGTGGAACTGGA TGGTGATGTGAACGGCCATAAATTTAGCGTGAGCGGCGAAGGCGAAGGTG ATGCGACCTATGGCAAACTGACCCTGAAACTGATTTGCACCACCGGCAAA CTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCTGGGCTATGGCCTGCA ATGCTTTGCGCGTTATCCGGATCATATGAAACAGCACGATTTCTTTAAAA GCGCCATGCCGGAAGGCTATGTGCAGGAACGCACCATCTTTTTTAAAGAT GATGGCAACTATAAAACCCGTGCGGAAGTGAAATTTGAAGGCGATACCCT GGTGAACCGTATTGAACTGAAAGGCATCGATTTCAAAGAAGATGGCAACA TTCTGGGCCATAAACTGGAATATAACTACAACAGCCATAACGTGTATATC ACCGCGGATAAACAGAAAAACGGCATCAAAGCGAACTTTAAAATCCGCCA CAACATTGAAGATGGCGGCGTGCAGCTGGCCGATCATTATCAGCAGAACA CCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGATAACCATTATCTGAGC TACCAGAGCAAACTGAGCAAAGATCCGAACGAAAAACGTGACCATATGGT GCTGCTGGAATTTGTGACCGCGGCCGGTATTACCCATGGCATGGATGAAC TGTATAAAACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKTG

    Commented GenBank file

  • FokA+YFP(Venus)_pEX.txt



    •  Longlinker+FokA_pMA

    back to index

    Longlinker+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGG CAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAAT ATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACC CAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTA TGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCA TTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAA GCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCA GCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACG AATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTC GTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAA CCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGA TTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGC CGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYV KENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVE ELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Longlinker+FokA_pMA.txt



    •  Longlinker+Foki_pMA

    back to index

    Longlinker+Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGG CAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAAT ATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACC CAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTA TGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCA TTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAA GCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGA ACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACG AATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTC GTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAA CCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGA TCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGC CGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM EFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYV EENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVE ELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Longlinker+Foki_pMA.txt



    •  middelLinker+FokA_pMA

    back to index

    middelLinker+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACT GGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACG AGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATC CTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGG CAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTG GTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGG GGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAA AGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAG TGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCAT TTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAA TAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAA TGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAAC AATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQ TRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • middelLinker+FokA_pMA.txt



    •  ShortLinker26+FokA_pMA

    back to index

    ShortLinker26+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYG YRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNK HINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEM IKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • ShortLinker26+FokA.txt



    •  Strep+Dig_pMA

    back to index

    Strep+Dig in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTG

    Commented GenBank file

  • Strep+Dig.txt



    •  Strep+FluA_pMA

    back to index

    Strep+FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTG

    Commented GenBank file

  • Strep+FluA.txt



    •  Split+Foki_pMA

    back to index

    Split+Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGC GCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATC GCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTT CTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTA AACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTT ATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCA GGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAAC ATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAG TTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCA GCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTG TTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACT CTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGG TTAA

    AA sequence of ORF-1:

    MAGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRIL EMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADE MERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGA VLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Split+Foki.txt



    •  His+FluA_pMA

    back to index

    His+FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTG

    Commented GenBank file

  • His+FluA.txt



    •  Fos+Split+FokA_pMA

    back to index

    Fos+Split+FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA AAAACTGGAGTTCATCCTGGCAGCAACCGGCCGACCAGCCTGTAAGATTC CAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAAATCTGAACTG GAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGA GTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCC TGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGC AAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGG TTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGG GTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAA GAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGT GTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATT TTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAAT AGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAAT GATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACA ATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA NLLKEKEKLEFILAATGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Fos+Split+FokA_pMA.txt



    •  His+Dig+Split+FokA_pJS419

    back to index

    His+Dig+Split+FokA in pJS419 Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pJS419.txt





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    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning"