Team:Utah State

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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#A9A9A9">Home</font>]]
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]]
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        <td width="172" id="ana"><span class="currentPage"><font size = 4>HOME</font></span>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Team"><font size = 4>ABOUT US</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Project"><font size = 4>PROJECT</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>BIOBRICKS</font></a></td>
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      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
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              <b><i>Welcome!</b></i></font>
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The Utah State University team is happy to have received a GOLD MEDAL at the 2009 iGEM Jamboree. We are very proud of all that we were able to accomplish this year. We invite you to explore our site and learn all about our project! And please contact us if you would like more information about any aspect of our project.</p>
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<p class = "class"> The aim of the Utah State University iGEM project is to develop improved production and harvesting methods of proteins and other products in multiple organisms using the standardized BioBrick system. The name of our project, BioBricks without Borders, characterizes and ties together the two main focuses of our research:</p></br>
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<li>Investigating broad host-range vectors for production of compounds in organisms other than <i>E. coli</i> (like <i>Synechocystis</i> PCC6803, <i>Rhodobacter sphaeroides</i>, and <i>Pseduomonas putida</i>)
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<li>Developing a library of fusion-compatible BioBrick parts for targeting compounds for secretion</li>
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    <h1>Overview</h1>
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    <b>Since the emergence of Synthetic Biology</b>, bacteria have been engineered to perform a wide variety of simple tasks. They can be made to express proteins, respond to their environment and communicate primitively with each other. Presently, a key goal for the field is to create a communicating, organised and differentiated population of bacteria that can be considered a multicellular organism, capable of performing even more complex tasks. The ultimate goal for this line of research would be to mimic a brain, the most complex structure in the universe. To realize this goal requires the development of systems for rapid, robust communication and self-organised differentiation. <br /> <b> Our project sets the foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial patterning.</b>
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          <h1>Voltage</h1>
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In order to simulate neural activity in bacteria, a mechanism resembling a synapse is necessary. At the synapse, neurotransmitter molecules are released from the presynaptic plasma membrane. The neurotransmitter diffuses through the synaptic cleft and binds to chemical receptor molecules on the membrane of the postsynaptic cell. These receptors cause ion channels to open so that ions rush out, changing the transmembrane potential. Attempting to mimic this in a prokaryotic system is particularly attractive as, in a more general sense, it provides an interface between chemical or biological and electrical systems. <br /><b> Using the amino acid glutamate as our 'neurotransmitter', we have successfully demonstrated a voltage response in bacterial cells. </b> <a href="https://2008.igem.org/Team:Cambridge/Voltage"> Read on...</a>
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          <h1>Signalling</h1>
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Using peptide-based signalling systems from gram-positive bacteria, we have laid the foundations for a self-organising biological system, capable of expressing spatial patterns of GFP expression on a bacterial lawn. The focus of our investigation was on a simple two-component Reaction-Diffusion system, allowing for simple spatial 'patterning' of gene expression. The simplest of these patterns mimic the spots and stripes seen on animal coats. In 1952, Alan Turing famously described this Reaction-Diffusion system and suggested it as the basis for self-organization and pattern formation in biological systems. <br /><b> This is a first step in the direction of engineering multicellular behaviour. </b> <a href="https://2008.igem.org/Team:Cambridge/Signalling"> Read on...</a>
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          <h1>Bacillus</h1>
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To build more complex cellular systems, new tools and techniques are required. We are generating standardized parts, tools, and techniques for the gram-positive chassis ''B. subtillis''. Easy to handle and transform, this bacterium offers many adantages to ''E. coli', including the ability to secrete proteins and integrate DNA into the chromosome. We have designed, built, and submitted gram-positive RBSes, promoters, and shuttle vectors.<br /> <b> As a part of this work we have confirmed single copy chromosomal insertion, demonstrated InFusion assembly, and characterized an improved GFP variant. </b>
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<a href="https://2008.igem.org/Team:Cambridge/Bacillus"> Read on...</a>
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We have introduced a model of the AGR quorum-sensing system of S.aureus to illustrate how a typical quorum-sensing system works. The model predicts theoretical values for biological parameters (such as the threshold cell density) that can be verified and we will be also be able to predict how the system behaves if we change a number crucial parameters. This can be extremely useful in informing design decisions when building a synthetic device. We have also expanded this model into a hypothetical setup with a second parallel agr-system. <br /><b> Using this parallel signals model, we investigate how to engineer a biological patterning system.</b> <a href="https://2008.igem.org/Team:Cambridge/Modelling">Read on...</a>
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''In the interest of creating a universal BioBrick vector, the goal of our project is to improve, modify, and test the broad-host vector pRL 1383a. After ensuring that the vector functions properly, we will test in the cyanobacterium Synechocystis, Pseudomonas putida, and Escherichia coli. We will then develop expression systems in Synechocystis to produce various proteins. ''
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Latest revision as of 04:13, 12 November 2009

USU iGem Untitled Document

HOME
Welcome!

The Utah State University team is happy to have received a GOLD MEDAL at the 2009 iGEM Jamboree. We are very proud of all that we were able to accomplish this year. We invite you to explore our site and learn all about our project! And please contact us if you would like more information about any aspect of our project.

The aim of the Utah State University iGEM project is to develop improved production and harvesting methods of proteins and other products in multiple organisms using the standardized BioBrick system. The name of our project, BioBricks without Borders, characterizes and ties together the two main focuses of our research:


  • Investigating broad host-range vectors for production of compounds in organisms other than E. coli (like Synechocystis PCC6803, Rhodobacter sphaeroides, and Pseduomonas putida)
  • Developing a library of fusion-compatible BioBrick parts for targeting compounds for secretion



Retrieved from "http://2009.igem.org/Team:Utah_State"