From 2009.igem.org
(Difference between revisions)
|
|
| (One intermediate revision not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:BCCS-Bristol/Header}}
| |
| | | | |
| - |
| |
| - | ===Week 6===
| |
| - |
| |
| - | * XL1-Blue transformations with ligations 1 and 2 from Week 5.
| |
| - |
| |
| - | * Restriction digests for FhuA and OsmE to prepare their insertion downstream of the AraC-RBS costruct. Also GFP cut to fuse it with BOO14 terminator.
| |
| - |
| |
| - | * Ligation set up between GFP and B0014 terminator (on pSB1AK3) and used to transform XL1-Blue.
| |
| - |
| |
| - | * Transformations with AraC-RBS and pQE31-GFP didn't work. Repeated with NovaBlue cells.
| |
| - |
| |
| - | * AraC-RBS transformations worked very well in contrast with pQE31-GFP and GFP-terminator that didn't work again.
| |
| - |
| |
| - | * Repeat ligations between pQE31-GFP and GFP-terminator and transform XL1-Blue.
| |
| - |
| |
| - | * Minipreps and Restr. Digests for AraC-RBS constructs.
| |
| - |
| |
| - | * pQE31-GFP and GFP-terminator transformations failed.
| |
| - |
| |
| - | * Ligations set up:
| |
| - | 1. pQE31+GFP
| |
| - | 2. AraC-RBS + FhuA
| |
| - | 3. AraC-RBS + OsmE
| |
| - | 4. GFP-terminator
| |
| - |
| |
| - |
| |
| - | [[Image:BCCS_Promoter_rbs_presence.jpg|center|100px|frame|1kb NEB Standard Ladder. Lanes run in triplets featuring Double Digest (XbaI/SpeI), Single Digest(XbaI) and uncut versions of DNA plasmid carrying the AraC-RBS ligation product. The product is seen as a single band at the bottom of the Double Digest lanes. The top band is the pSB1A2 Carrier plasmid.]]
| |
| - |
| |
| - |
| |
| - | {{:Team:BCCS-Bristol/NotebookHeader}}
| |
Latest revision as of 23:48, 20 October 2009
"
