Team:Utah State

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    <td bgcolor="#231f20" align="center"><a href="https://2009.igem.org/Team:Utah_State/Project">PROJECT</a></td>
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        <td width="172" id="ana"><span class="currentPage"><font size = 4>HOME</font></span>
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               <b><i>BioBricks without Borders:</b></i></font>
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               <b><i>Welcome!</b></i></font>
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               <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR>
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<p> <font size="2.5" face=" Tahoma, Helvetica, Arial" color =#000000>The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like <i>Pseudomonas putida</i>, <i>Rhodobacter sphaeroides</i>, and <i>Synechocystis</i> PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation.  Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense.  To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions.  To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.</p>
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The Utah State University team is happy to have received a GOLD MEDAL at the 2009 iGEM Jamboree. We are very proud of all that we were able to accomplish this year. We invite you to explore our site and learn all about our project! And please contact us if you would like more information about any aspect of our project.</p>
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            <p>  <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
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<p class = "class"> The aim of the Utah State University iGEM project is to develop improved production and harvesting methods of proteins and other products in multiple organisms using the standardized BioBrick system. The name of our project, BioBricks without Borders, characterizes and ties together the two main focuses of our research:</p></br>
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<li>Investigating broad host-range vectors for production of compounds in organisms other than <i>E. coli</i> (like <i>Synechocystis</i> PCC6803, <i>Rhodobacter sphaeroides</i>, and <i>Pseduomonas putida</i>)
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<li>Developing a library of fusion-compatible BioBrick parts for targeting compounds for secretion</li>
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Latest revision as of 04:13, 12 November 2009

USU iGem Untitled Document

HOME
Welcome!

The Utah State University team is happy to have received a GOLD MEDAL at the 2009 iGEM Jamboree. We are very proud of all that we were able to accomplish this year. We invite you to explore our site and learn all about our project! And please contact us if you would like more information about any aspect of our project.

The aim of the Utah State University iGEM project is to develop improved production and harvesting methods of proteins and other products in multiple organisms using the standardized BioBrick system. The name of our project, BioBricks without Borders, characterizes and ties together the two main focuses of our research:


  • Investigating broad host-range vectors for production of compounds in organisms other than E. coli (like Synechocystis PCC6803, Rhodobacter sphaeroides, and Pseduomonas putida)
  • Developing a library of fusion-compatible BioBrick parts for targeting compounds for secretion



Retrieved from "http://2009.igem.org/Team:Utah_State"