Team:Illinois/MicC

From 2009.igem.org

(Difference between revisions)

Latest revision as of 02:43, 22 October 2009

Home

Team

Project

Experiments

Biobricks Notebook

Back to sRNA Library Team page

MicC Target-GFP Fusion

Primers Used:

June 15

PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product.

June 16

Another PCR of the micC gene was done. Results should be analyzed tomorrow.

June 17

We performed a restriction enzyme digest of our small RNA and its target sequence. We then ran a gel to purify the DNA.

@@ Line 2: / Line 2: @@
 {{Illinois}}
-== '''MicC Target-GFP Fusion''' ==
+[https://2009.igem.org/Team:Illinois/sRNA_Library Back to sRNA Library Team page]
-'''People in Group:''' Phil Bell, Sonam Tanna, Davel Patel
+<html>
+<div id="uicontentbox">
+</html>
-'''Purpose:''' We want to fuse the MicC target sequence from the OmpC gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicC gene.  We expect to see translational repression of GFP by the small RNA MicC.
+== '''MicC Target-GFP Fusion''' ==
-'''Protocol(s) Used:''' (links to protocols page)
-'''Recipe(s) Used:'''
 '''Primers Used:'''