Team:Waterloo

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== '''Team Waterloo 2009 Project:<br> <i>Chromobricks: A Platform for Chromosome Engineering with BioBricks</i> ''' ==
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
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The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the <i>E. coli</i> genome. Six pairs of integrase-targeted <i>att</i> sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing <i>att</i> sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.
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[[Image:UWiGEMF09teampictures800px.JPG|thumb|center|x500px|frame|Those who showed up for picture day]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Waterloo | Team Example]]
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!align="center"|[[Team:Waterloo|Home]]
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!align="center"|[[Team:Waterloo/Team|The Team]]
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!align="center"|[[Team:Waterloo/Project|The Project]]
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!align="center"|[[Team:Waterloo/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Waterloo/Modeling|Modeling]]
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Latest revision as of 21:39, 21 October 2009

Team Waterloo 2009 Project:
Chromobricks: A Platform for Chromosome Engineering with BioBricks

The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.


Those who showed up for picture day

Supported by UWiGEMSciFac.png

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