Team:Waterloo

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!align="center"|[[Team:Waterloo|Home]]
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== '''Team Waterloo 2009 Project:<br> <i>Chromobricks: A Platform for Chromosome Engineering with BioBricks</i> ''' ==
== '''Team Waterloo 2009 Project:<br> <i>Chromobricks: A Platform for Chromosome Engineering with BioBricks</i> ''' ==
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[[Image:UWiGEMF09teampictures800px.JPG|thumb|center|x500px|frame|Those who showed up for picture day]]
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Supported by [[Image:UWiGEMSciFac.png]]

Latest revision as of 21:39, 21 October 2009

Team Waterloo 2009 Project:
Chromobricks: A Platform for Chromosome Engineering with BioBricks

The aim of our project is to develop a fully-featured platform for chromosome engineering, allowing the in vivo assembly of a synthetic chromosome from interchangeable parts, followed by selective degradation of the native chromosome. We have designed a proof-of-concept for chromosome-building that will use the site-specific integrase of phage ΦC31 to integrate a BioBrick into a defined locus of the E. coli genome. Six pairs of integrase-targeted att sites have been designed to be non-cross-reactive in order to support repeatable cassette-exchange reactions for chromosome building. We have also written software to model the integrase-mediated rearrangement of DNA molecules containing att sites, to aid the design of more elaborate chromosome-building systems. To selectively degrade the native chromosome we designed a nuclease-based, inducible genome-degradation system. In its simplest form, our system can be used to integrate biological devices into a chromosome in situations requiring stable copy number and selection-free maintenance.


Those who showed up for picture day

Supported by UWiGEMSciFac.png

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