Team:UNIPV-Pavia/Notebook/Week4Sep

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<div>
<div>
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<html><a name="week_start"></a></html>
= Week from September 21st, to September 27th, 2009 =
= Week from September 21st, to September 27th, 2009 =
<html>
<html>
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">Previous Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">Next Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">Next Week</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
</a>
</a>
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== <html><font class="dayw_style">September, 21st</font></html> ==
== <html><font class="dayw_style">September, 21st</font></html> ==
 +
*Wiki updating
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 22nd</font></html> ==
== <html><font class="dayw_style">September, 22nd</font></html> ==
===== pH sensor =====
===== pH sensor =====
-
*LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation
+
*LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation.
* Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
* Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
**RBS33
**RBS33
**A2
**A2
-
**pNhaA + GFP (from now on it we'll be indicated only as pNhaA)
+
**pNhaA
-
* Overnight incubation of the three culctures.  
+
* Overnight incubation of these three cultures at 37°C, 220 rpm.
 +
 
 +
 
 +
''Experiment with Tecan F200''
 +
*<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/T9002 TEST 22-09-09.pdf" target="_blank">Download Protocol</a></html>
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 23rd</font></html> ==
== <html><font class="dayw_style">September, 23rd</font></html> ==
===== pH sensor =====
===== pH sensor =====
-
2nd experiment
+
''2nd experiment''
-
*We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half.
+
*We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
-
*OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
+
*OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
*We diluted:
*We diluted:
**RBS into:
**RBS into:
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*** LB NaCl 70 mM + Amp pH 7,5
*** LB NaCl 70 mM + Amp pH 7,5
*** LB NaCl 70 mM + Amp pH 8,5
*** LB NaCl 70 mM + Amp pH 8,5
-
*We started this 6 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
+
*We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
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== <html><font class="dayw_style">September, 24th</font></html> ==
== <html><font class="dayw_style">September, 24th</font></html> ==
-
===== pH sensor =====
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*Fermentation experiment setup
-
Qui risultati?
+
*Wiki updating
-
 
+
<div align="right">
<div align="right">
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*Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
*Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
*Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
*Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
-
*Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
 
-
**RBS33
 
-
**A2
 
-
**pNhaA
 
-
*Overnight incubation of the three cultures.
 
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== <html><font class="dayw_style">September, 26th</font></html> ==
== <html><font class="dayw_style">September, 26th</font></html> ==
-
 
+
*Fermentation experiment
 +
*Cloning
 +
*Wiki updating
<div align="right">
<div align="right">
[[#top|Top]]
[[#top|Top]]
</div>
</div>
-
== <html><font class="dayw_style">September, 27th</font></html> ==
 
 +
== <html><font class="dayw_style">September, 27th</font></html> ==
 +
===== pH sensor =====
 +
*Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
 +
**RBS33
 +
**A2
 +
**pNhaA
 +
*Overnight incubation of these three cultures at 37°C, 220 rpm.
<div align="right">
<div align="right">
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">Previous Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">Next Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">Next Week</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
</a>
</a>

Latest revision as of 23:39, 21 October 2009

EthanolPVanimation.gif

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July 2009
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August 2009
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September 2009
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Week from September 21st, to September 27th, 2009

Previous Week Next Week

September, 21st

  • Wiki updating

Top

September, 22nd

pH sensor
  • LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation.
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of these three cultures at 37°C, 220 rpm.


Experiment with Tecan F200

Top

September, 23rd

pH sensor

2nd experiment

  • We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
  • OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
    • pNhaA into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
    • A2 into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
  • We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


Top

September, 24th

  • Fermentation experiment setup
  • Wiki updating

Top

September, 25th

pH sensor
  • Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
  • Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.


Top

September, 26th

  • Fermentation experiment
  • Cloning
  • Wiki updating

Top

September, 27th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of these three cultures at 37°C, 220 rpm.

Top


Previous Week Next Week
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