Team:Tsinghua/Design2

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Latest revision as of 17:03, 21 October 2009

Home	Background	Brainstorming	Design	Experiment	Results	Conclusion	Protocol

Synthesis of Therapeutic DNA

therapeutic DNA construct

Here we introduce another molecular cloning vector to mimic the bacteriophage lambda genome which will be packaged into the virion. What’s more, the synthetic “bacteriophage lambda genome” contains the therapeutic gene(s) needed by the target cells, which will be transported via simulated viral introduction.

We term this molecular cloning vector “Therapeutic DNA”, which consists of a cos site for the package into the virion.The therapeutic gene is for in vivo gene therapy and owns the replication origin of bacteriophage lambda (including O gene and P gene).

@@ Line 1: / Line 1: @@
-==Synthesis of the Therapeutic DNA==
+{{Tsinghua/ProjectHeader}}
-[[Image:cosmid.png|500px|center]]
-Here we introduce another molecular cloning vector to mimic the bacteriophage lambda genome which will be packaged into the viroin. However, the synthetic “bacteriophage lambda genome” contains the therapeutic gene(s) needed by the target cells, which will be transported via stimulated viral introduction.
-We term this molecular cloning vector “Therapeutic DNA”, which consists of a cos site for the package into the viroin of targeted gene therapy vector, the therapeutic gene(s) for in vivo gene therapy and the replication origin of bacteriophage lambda (including O gene and P gene).
+==Synthesis of Therapeutic DNA==
+[[Image:cosmid.png|600px|center|thumb|therapeutic DNA construct]]
+Here we introduce another molecular cloning vector to mimic the bacteriophage lambda genome which will be packaged into the virion. What’s more, the synthetic “bacteriophage lambda genome” contains the therapeutic gene(s) needed by the target cells, which will be transported via simulated viral introduction.
+We term this molecular cloning vector “Therapeutic DNA”, which consists of a cos site for the package into the virion.The therapeutic gene is for in vivo gene therapy and owns the replication origin of bacteriophage lambda (including O gene and P gene).