"
Team:Imperial College London/Wetlab/Results/Thermoinduction/Harvard
From 2009.igem.org
| Line 1: | Line 1: | ||
{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
| + | {{Imperial/09/Tabs/Main/Wetlab/Protocols}} | ||
| + | In the experiment, 3 tests were temperature and fluorescence output was recorded for:<br> | ||
| + | *Cells with the Harvard Biobrick | ||
| + | *Negative control | ||
| + | *Positive control | ||
[[Image:II09_HVD1.jpg|700px]] | [[Image:II09_HVD1.jpg|700px]] | ||
| - | + | <b>Figure 1: Fluorescence trend for cells at 3 different temperatures (degrees celsius) with a negative and positive control</b><br> | |
| - | [[Image:II09_HVD2.jpg]] | + | From figure 1, no immediate trend is apparent. |
| + | However, if we study figure 2 more closely without looking at the controls, we can see that as temperature is increased, fluorescence count increases too. <br> | ||
| + | However, there are several artefacts in the data. | ||
| + | [[Image:II09_HVD2.jpg]]<br> | ||
| + | <b>Figure 2: Fluorescence trend for cells at 3 different temperatures (degrees celsius)</b> <br><br> | ||
| + | <b>Conclusion:</b> | ||
| + | When temperature increases, the repression of the Harvard promoter is lifted and genome deletion by thermoinduction can take place. This can be observed by a higher fluorescence measure when the temperature rises. | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 11:18, 12 October 2009
In the experiment, 3 tests were temperature and fluorescence output was recorded for:
- Cells with the Harvard Biobrick
- Negative control
- Positive control
Figure 1: Fluorescence trend for cells at 3 different temperatures (degrees celsius) with a negative and positive control
From figure 1, no immediate trend is apparent.
However, if we study figure 2 more closely without looking at the controls, we can see that as temperature is increased, fluorescence count increases too.
However, there are several artefacts in the data.
Figure 2: Fluorescence trend for cells at 3 different temperatures (degrees celsius)
Conclusion:
When temperature increases, the repression of the Harvard promoter is lifted and genome deletion by thermoinduction can take place. This can be observed by a higher fluorescence measure when the temperature rises.
