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Team:Berkeley Wetlab/Recipes
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====10X TBST==== | ====10X TBST==== | ||
| - | 31.4 g TRIS HCl | + | 31.4 g TRIS HCl<br> |
| + | 80g NaCl<br> | ||
| + | 10ml Tween-20<br> | ||
| + | bring volume to 1000ml<br> | ||
| + | pH 7.5<br> | ||
| + | |||
| + | ====1X TBS==== | ||
| + | 605 mg Tris<br> | ||
| + | 876 mg NaCl<br> | ||
| + | 80 ml H2O<br> | ||
| + | pH 7.4<br> | ||
| + | bring volume to 100 ml<br> | ||
| + | |||
| + | ====Artificial Seawater==== | ||
| + | 500 ml ddH2O<br> | ||
| + | 12.2 g NaCl<br> | ||
| + | 2.6 g MgCl2<br> | ||
| + | .57 g CaCl2<br> | ||
| + | |||
| + | ====Tris Buffer + 2% SDS==== | ||
| + | 50 mM Tris<br> | ||
| + | 150 mM NaCl<br> | ||
| + | 2% SDS<br> | ||
| + | pH 7<br> | ||
| + | |||
| + | ====Precipitation Buffer==== | ||
| + | 2 mL of acetic acid<br> | ||
| + | Add concentrated NaOH until the pH is 5<br> | ||
| + | Add water to reach 10 mL total volume volume<br> | ||
| + | 0.2 g ZnCl<br> | ||
| + | Add 40 mL ethanol<br> | ||
| + | |||
| + | |||
| + | '''*For our Assay Protocols Visit the Passengers Tab''' | ||
Latest revision as of 20:49, 18 October 2009
|
Contents |
10X TBST
31.4 g TRIS HCl
80g NaCl
10ml Tween-20
bring volume to 1000ml
pH 7.5
1X TBS
605 mg Tris
876 mg NaCl
80 ml H2O
pH 7.4
bring volume to 100 ml
Artificial Seawater
500 ml ddH2O
12.2 g NaCl
2.6 g MgCl2
.57 g CaCl2
Tris Buffer + 2% SDS
50 mM Tris
150 mM NaCl
2% SDS
pH 7
Precipitation Buffer
2 mL of acetic acid
Add concentrated NaOH until the pH is 5
Add water to reach 10 mL total volume volume
0.2 g ZnCl
Add 40 mL ethanol
*For our Assay Protocols Visit the Passengers Tab
