Team:IPN-UNAM-Mexico/Notebook

From 2009.igem.org

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=NOTEBOOK=
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<!-- {{{Año}}}/06 (Junio) -->
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{| 
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/June|<font color="#D9D9F3">June</font>]]</Center>
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|- bgcolor="#BCD2EE"
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As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into ''E. coli''. For this reason we had to delay lab work and request the 2009 catalog.
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Protocols|Protocols]]</h1>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/July|July]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/August|August]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/September|September]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/October|October]]</h2>
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Safety|Safety]]</h1>
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Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is  described below:
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<center>
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{| border="2"
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!Number
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!Biobrick
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!Restriction
|-
|-
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| &nbsp;1||&nbsp;2 || &nbsp;3 || &nbsp;&nbsp;4 || &nbsp;5 || &nbsp;6 || &nbsp;7 ||
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|2
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|BBa_R0079
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|S & P
|-
|-
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| &nbsp;8 ||&nbsp;9 || 10 || &nbsp;11 || 12 || 13 || 14 ||
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|3
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|BBa_F1610
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|X & P
|-
|-
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| 15||16 || 17 || &nbsp;18 || 19 || 20 || 21 ||
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|4
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|BBa_K091146
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|S & P
|-
|-
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| 22||23 || 24 || &nbsp;25 || 26 || 27 || 28 ||
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|5
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|BBa_K093005
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|X &  
|-
|-
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| 29||30 || &nbsp;<Font Color="#c0c0c0">1</Font> || &nbsp;&nbsp;<Font Color="#c0c0c0">2</Font> || &nbsp;<Font Color="#c0c0c0">3</Font> || &nbsp;<Font Color="#c0c0c0">4</Font> || &nbsp;<Font Color="#c0c0c0">5</Font>
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|6
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|}
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|BBa_K081016
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|| || valign="top" |
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|X & P
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<!-- {{{Año}}}/07 (Julio) -->
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{| 
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/July|<font color="#D9D9F3">July</font>]]</Center>
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|- bgcolor="#BCD2EE"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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|-
|-
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| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;1 || &nbsp;2 || &nbsp;3 || &nbsp;4 || &nbsp;5 ||
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|7
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|BBa_EC840
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|X & P
|-
|-
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| &nbsp;6||&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_7_2009#July 7th|7]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_8_2009#July 8th|8]] || &nbsp;&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July_9_2009#July 9th|9]] || [[Team:IPN-UNAM-Mexico/Notebook/July_10_2009#July 10th|10]] || [[Team:IPN-UNAM-Mexico/Notebook/July_11_2009#July 11th|11]] || 12 ||
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|8
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|BBa_K081009
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|X & P
|-
|-
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| 13 ||[[Team:IPN-UNAM-Mexico/Notebook/July#July 14th|14]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 15th|15]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 16th|16]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 17th|17]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 18th|18]] || 19 ||
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|9
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|BBa_R0051
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|S &P
|-
|-
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| 20||[[Team:IPN-UNAM-Mexico/Notebook/July#July 21st|21]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 22nd|22]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 23rd|23]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 24th|24]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 25th|25]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 26th|26]] ||
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|10
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|BBa_J06800
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|X & P
|-
|-
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| 27||[[Team:IPN-UNAM-Mexico/Notebook/July#July 28th|28]]|| [[Team:IPN-UNAM-Mexico/Notebook/July#July 29th|29]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/July#July 30th|30]] || [[Team:IPN-UNAM-Mexico/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
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|11
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|}
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|BBa_Q04121
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|}
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|X & P
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{|
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<!-- {{{Año}}}/08 (Agosto) -->
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{| 
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/August|<font color="#D9D9F3">August</font>]]</Center>
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|- bgcolor="#BCD2EE"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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|-
|-
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| <Font Color="#c0c0c0">27</Font> || <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">29</Font> || &nbsp;<Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August #August 1st|1]] || &nbsp;2 
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|12
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|BBa_B0034
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|S & P
|-
|-
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| &nbsp;3 ||&nbsp;4 || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 5th|5]] || &nbsp;&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August_6_2009#August 6th|6]] ||&nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August_7_2009#August 7th|7]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August_8_2009#August 8th|8]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August_9_2009#August 9th|9]]
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|13
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|BBa_C0079
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|E & S
|-
|-
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| [[Team:IPN-UNAM-Mexico/Notebook/August_10_2009#August 10th|10]] || [[Team:IPN-UNAM-Mexico/Notebook/August_11_2009#August 11th|11]] || [[Team:IPN-UNAM-Mexico/Notebook/August_12_2009#August 12th|12]] || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August_13_2009#August 13th|13]]|| [[Team:IPN-UNAM-Mexico/Notebook/August_14_2009#August 14th|14]]|| [[Team:IPN-UNAM-Mexico/Notebook/August#August 15th|15]] || 16
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|14
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|BBa_C0179
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|E & S
|-
|-
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|17|| [[Team:IPN-UNAM-Mexico/Notebook/August#August 18th|18]] || 19 || &nbsp;20 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 21st|21]] || 22 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 23rd|23]]
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|15
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|BBa_B0015
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|E & X
|-
|-
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| 24||[[Team:IPN-UNAM-Mexico/Notebook/August#August 25th|25]] || 26 || &nbsp;[[Team:IPN-UNAM-Mexico/Notebook/August#August 27th | 27]] || 28 || [[Team:IPN-UNAM-Mexico/Notebook/August#August 29th | 29 ]] || [[Team:IPN-UNAM-Mexico/Notebook/August#August 30th | 30]]
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|16
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|BBa_K081018
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|E & S
|-
|-
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|31||<Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font> || <Font Color="#c0c0c0">5</Font> || <Font Color="#c0c0c0">6</Font>
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|17
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|}
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|BBa_K116640
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|X & P
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<!-- {{{Año}}}/09 (Septiembre) -->
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{|
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/September|<font color="#D9D9F3">September</font>]]</Center>
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|- bgcolor="#BCD2EE"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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|-
|-
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| <Font Color="#c0c0c0">31</Font>||&nbsp;1 || &nbsp;2 || &nbsp;&nbsp;3 || &nbsp;4 || &nbsp;5 || &nbsp;6||
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|23
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|BBa_S03154
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|X & P
|-
|-
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| &nbsp;7||&nbsp;8 || &nbsp;9 || &nbsp;10 || 11 || 12 || 13 ||  
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|24
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|BBa_k145201
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|E & S
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|}
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</center>
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<center>
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{| border="2"
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! Ligation
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! Nickname
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! Biobrick
|-
|-
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| 14 ||15 || 16 || &nbsp;17 || 18 || 19 || 20 ||  
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|4-23
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|Ciencias I
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|BBa_K226005
|-
|-
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| 21||22 || 23 || &nbsp;24 || 25 || 26 || 27 ||  
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|13-15
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|Ciencias II
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|BBa_K266002
|-
|-
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| 28||29 || 30 || &nbsp;<Font Color="#c0c0c0">1</Font> || <Font Color="#c0c0c0">2</Font> || <Font Color="#c0c0c0">3</Font> || <Font Color="#c0c0c0">4</Font>
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|Ciencias I-7
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|}
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|Ciencias III
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|BBa_K266006
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<!-- {{{Año}}}/10 (Octubre) -->
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{| 
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/October|<font color="#D9D9F3">October</font>]]</Center>
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|- bgcolor="#BCD2EE"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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|-
|-
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| <Font Color="#c0c0c0">28</Font> ||<Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp; 1|| &nbsp;2 || &nbsp;3 || &nbsp;4
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|24-17
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|Ciencias IV
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|BBa_K266001
|-
|-
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| &nbsp;5||&nbsp;6 || &nbsp;7  || &nbsp;&nbsp;8|| 9 || 10 || 11||  
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|2-3
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|Ciencias V
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|BBa_K266000
|-
|-
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| 12||13 || 14  || &nbsp;15 || 16 || 17 || 18 ||  
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|J231000-11
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|Ciencias VI
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|BBa_K266008
|-
|-
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| 19||20 || 21 || &nbsp;22 || 23  || 24  || 25 ||  
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|Amplicon - Ciencias II
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|Ciencias VII
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|BBa_K266003
|-
|-
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| 26|| 27  || 28 || &nbsp;29 || 30 || 31 || <Font Color="#c0c0c0">&nbsp;1</Font>
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|Ciencias VI - 12
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|}
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|Ciencias VIII
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|| || valign="top" |
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|BBa_K266009
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<!-- {{{Año}}}/11 (Noviembre) -->
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{|  
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| colspan="7" style="background:#23238E;" | <Center>[[Team:IPN-UNAM-Mexico/Notebook/November|<font color="#D9D9F3">November</font>]]</Center>
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|- bgcolor="#BCD2EE"
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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|-
|-
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| <Font Color="#c0c0c0">26</Font> ||<Font Color="#c0c0c0">27</Font> || <Font Color="#c0c0c0">28</Font> || <Font Color="#c0c0c0">&nbsp;29</Font> || <Font Color="#c0c0c0">30</Font> || <Font Color="#c0c0c0">31</Font> || &nbsp;1
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|Ciencias III - 19
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|Ciencias IX
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|BBa_K266006
|-
|-
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| &nbsp;2||&nbsp;3 || &nbsp;4 || &nbsp;&nbsp;5 || &nbsp;6 || &nbsp;7|| &nbsp;8
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|J23100 – Ciencias VII
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|Ciencias X
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|BBa_K266004
|-
|-
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| &nbsp;9||10 || 11 || &nbsp;12 || 13 || 14 || 15 ||  
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|Ciencias III - Ciencias V
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|Ciencias XI
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|BBa_K266007
|-
|-
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| 16||17 || 18 || &nbsp;19 || 20 || 21 || 22 ||
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|Ciencias X - Ciencias IV
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|-
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|Ciencias XII
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| 23 ||24 || 25 || &nbsp;26 || 27 || 28 || 29
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|BBa_K266010
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|-
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|}</center>
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| 30 ||<Font Color="#c0c0c0">&nbsp;1</Font> ||<Font Color="#c0c0c0">&nbsp;2</Font> ||<Font Color="#c0c0c0">&nbsp;3</Font> ||<Font Color="#c0c0c0">&nbsp;4</Font> ||<Font Color="#c0c0c0">&nbsp;5</Font> ||<Font Color="#c0c0c0">&nbsp;6</Font>  
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{{Template:IPN-UNAM-Mexico-footer}}
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<br><br><br><br><br><br>
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Latest revision as of 01:29, 22 October 2009


BannerUNAM.jpg

NOTEBOOK

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.

Month-icon.pngProtocols

Month-icon.pngJuly

Month-icon.pngAugust

Month-icon.pngSeptember

Month-icon.pngOctober

Month-icon.pngSafety

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:


Number Biobrick Restriction
2 BBa_R0079 S & P
3 BBa_F1610 X & P
4 BBa_K091146 S & P
5 BBa_K093005 X &
6 BBa_K081016 X & P
7 BBa_EC840 X & P
8 BBa_K081009 X & P
9 BBa_R0051 S &P
10 BBa_J06800 X & P
11 BBa_Q04121 X & P
12 BBa_B0034 S & P
13 BBa_C0079 E & S
14 BBa_C0179 E & S
15 BBa_B0015 E & X
16 BBa_K081018 E & S
17 BBa_K116640 X & P
23 BBa_S03154 X & P
24 BBa_k145201 E & S


Ligation Nickname Biobrick
4-23 Ciencias I BBa_K226005
13-15 Ciencias II BBa_K266002
Ciencias I-7 Ciencias III BBa_K266006
24-17 Ciencias IV BBa_K266001
2-3 Ciencias V BBa_K266000
J231000-11 Ciencias VI BBa_K266008
Amplicon - Ciencias II Ciencias VII BBa_K266003
Ciencias VI - 12 Ciencias VIII BBa_K266009
Ciencias III - 19 Ciencias IX BBa_K266006
J23100 – Ciencias VII Ciencias X BBa_K266004
Ciencias III - Ciencias V Ciencias XI BBa_K266007
Ciencias X - Ciencias IV Ciencias XII BBa_K266010

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