Team:IPN-UNAM-Mexico/Notebook/August 10 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 08:18, 14 October 2009

Back to Notebook

August 10th, 2009

Calculus for Mix; plasmid linearization.

For 20 reactions.

Mix	For every Eppendorf tube	For 20 reactions
PlasmidicDNA	3 μl	60 μl
Buffer 2	1 μl	20 μl
BSA	0.5μl	10 μl
EcoRI	0.5 μl / 5 μll	10 μl / 100 μl
H2O	5 μl / 10 μl vf	100 μl / 200 μl vf

Electrophoresis gel load:

3 μl of colorant
10 μl of digestion
3 μl of molecular weight marker

Load order.

Well Plasmid Well Plasmid

2          16,2”               13            3.3
3          4:23 L2            14            22.1
4          15:3 L2            15            19.2*
5          18.2*             16            24.2”
6          2.3

Well                             Well
1          1kb 2 μl         12            2.3
2          16.2”             13            14.2
3          4:23 L2          14            3.3
4          15:3 L2          15            22.1
5          18.2              15            19.1*
6          15.13

Horizontal Gene Transfer (HGT).
Acording to the model proposed to Arturo, we made a search for the candidate bioparts to make their elusion and transformation, which got in the next way:

2 Constructions:

Construction 1:
Biopart BBa_K145201

J23116 + B0034 + C0040 + B0010 + B0012

Construction 2:
The first option for the construction 2 is made up from two devices: BBa_J01101 and Bba_I763011. The second one consists in asking Boston for the construction BBa_I763027. We will tell Arturo about this posibility.
First option:
Device 1.
B0040 + B0034 + C0051 + B0010 + B0012
Device 2.
B0051 + B0034 + J04031

LOCATION

	Plate	Well	Plasmid
Device 1 (piece1)	2009 kitplate1	11H	PSB1A2
Device 2 (piece 2)	2009 kitplate1	15L	PSB1A2

@@ Line 70: / Line 70: @@
 							  <table width="100%" height=100%" "border="0" cellspacing="0" cellpadding="0">
                                  <tr>
-                                   <td><p>&nbsp;</p>
+                                   <td><p align="right">&nbsp;</p>
+                                    <p align="right"><img src="https://static.igem.org/mediawiki/2009/a/a6/LabIconMX.png" alt="LabNotebook" width="30" height="35"> <span><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook">Back to Notebook</a></span></p>
                                      <p><strong>August 10th, 2009</strong></p>
                                      <p>Calculus for Mix; plasmid linearization.</p>