"
Team:Washington/Future
From 2009.igem.org
(Difference between revisions)
(→Overview) |
|||
| (49 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
{{Template:Team:Washington/Templates/Header}} | {{Template:Team:Washington/Templates/Header}} | ||
| + | ='''Overview'''= | ||
*Target Construct | *Target Construct | ||
# Attempt to add additional proteins into the vector and test for functionality | # Attempt to add additional proteins into the vector and test for functionality | ||
# Vary linker lengths | # Vary linker lengths | ||
| - | # Make a simpler version for trouble shooting the secretion | + | # Make a simpler version for trouble shooting the secretion system: [6x-His]-[NheI]-[prtB] |
# Transfer to a vector with the same origin and resistance as described in the original secretion system (pBR322+Carb). | # Transfer to a vector with the same origin and resistance as described in the original secretion system (pBR322+Carb). | ||
# Add a lacI into the expression and target vectors so repression is hard-coded into the vector and expression can be induced regardless of the cell line. | # Add a lacI into the expression and target vectors so repression is hard-coded into the vector and expression can be induced regardless of the cell line. | ||
| Line 13: | Line 14: | ||
# Transfer to a Chlor resistance, p15A origin vector as used in the original papers | # Transfer to a Chlor resistance, p15A origin vector as used in the original papers | ||
# Add original upstream DNA (50bp) before the native RBS to ensure proper function | # Add original upstream DNA (50bp) before the native RBS to ensure proper function | ||
| - | # Add an | + | # Add an arabinose inducible promoter for better control over secretion system activation |
| - | # Combine with target vector so entire secretion system | + | # Combine with target vector so entire secretion system is contained in one plasmid. |
*Display System | *Display System | ||
| - | # | + | # [https://2009.igem.org/Team:Washington/Project/CDS Construct a new modular display system: CDS] |
| - | # | + | # [https://2009.igem.org/Team:Washington/Project/FoldIt Use computational protein design to create a monomeric protein that binds tightly to biotin: FoldIt] |
| + | |||
| + | |||
| + | '''Continue to [https://2009.igem.org/Team:Washington/Accomplishments Accomplishments and & Submitted BioBricks]''' | ||
Latest revision as of 04:09, 20 October 2009
Overview
- Target Construct
- Attempt to add additional proteins into the vector and test for functionality
- Vary linker lengths
- Make a simpler version for trouble shooting the secretion system: [6x-His]-[NheI]-[prtB]
- Transfer to a vector with the same origin and resistance as described in the original secretion system (pBR322+Carb).
- Add a lacI into the expression and target vectors so repression is hard-coded into the vector and expression can be induced regardless of the cell line.
- Secretion System
- Transfer to a Chlor resistance, p15A origin vector as used in the original papers
- Add original upstream DNA (50bp) before the native RBS to ensure proper function
- Add an arabinose inducible promoter for better control over secretion system activation
- Combine with target vector so entire secretion system is contained in one plasmid.
- Display System
- Construct a new modular display system: CDS
- Use computational protein design to create a monomeric protein that binds tightly to biotin: FoldIt
