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Team:BIOTEC Dresden/Methods Vesicles
From 2009.igem.org
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The microfluidic system consists of a flow chamber created out of a hardened polymer and a pump system that controls the rates of flow of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated by the liquid flow along a grid that allows temporary containment and imaging of the droplets. | The microfluidic system consists of a flow chamber created out of a hardened polymer and a pump system that controls the rates of flow of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated by the liquid flow along a grid that allows temporary containment and imaging of the droplets. | ||
| - | Production of flow chambers | + | Production of flow chambers: |
* mix PDMS and curing agent in 10:1 ratio | * mix PDMS and curing agent in 10:1 ratio | ||
* degas and pour on wafer with etched microstructures | * degas and pour on wafer with etched microstructures | ||
Revision as of 15:53, 15 October 2009
Vesicles - Methods
Setup of the microfluidic system
The microfluidic system consists of a flow chamber created out of a hardened polymer and a pump system that controls the rates of flow of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated by the liquid flow along a grid that allows temporary containment and imaging of the droplets.
Production of flow chambers:
- mix PDMS and curing agent in 10:1 ratio
- degas and pour on wafer with etched microstructures
- polymerize on heat plate at 150 ºC for 30 min
- add unpolymerized PDMS mixture to points on microstructure where microtube inlets are to be pierced
- polymerize on heat plate at 150 ºC for 30 minutes
- remove polymerized PDMS from wafer, cut to fit onto glass cover slide (24 x 60 mm), and use clean needles (0.8 mm) or laser cutter to pierce tube inlets
- ionize PDMS and glass slide in plasma chamber for 30 sec to make it reactive
- align PDMS on glass slide and seal
- seal irreversibly by heating on plate at 60ºC for 6 hours
