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HKU-HKBU/29 July 2009
From 2009.igem.org
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{{Team:HKU-HKBU/header}} | {{Team:HKU-HKBU/header}} | ||
| - | + | Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+ | |
| - | + | 14:10 Subculture to warm room (37℃), shaking for 1.5h. | |
| - | + | 15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for | |
| - | + | 15min. | |
| - | + | Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant | |
Revision as of 14:29, 16 October 2009
Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT C+② Lac Iq A+ 14:10 Subculture to warm room (37℃), shaking for 1.5h. 15:40 take the subculture bacteria out from the warm room.Freeze the bacteria in 0℃ ice water for 15min. Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant
