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Team:Groningen/Parts/Used Parts
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===Promotors=== | ===Promotors=== | ||
| - | '''<partinfo>J23100</partinfo> | + | '''<partinfo>J23100</partinfo> Constitutive promoter family member (high expression)''' <br> pHigh is a high consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. <partinfo>pSB3K3</partinfo>, <partinfo>BBa_J61002</partinfo> and <partinfo>pSB1AC3</partinfo> <br> '''[http://partsregistry.org/Part:BBa_J23100:Design| More Information]''' |
| - | '''<partinfo>J23101</partinfo> | + | '''<partinfo>J23101</partinfo> Constitutive promoter family member (high expression, reference)''' <br> pHigh is a high consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. <partinfo>pSB3K3</partinfo>, <partinfo>BBa_J61002</partinfo> and <partinfo>pSB1AC3</partinfo> <br> '''[http://partsregistry.org/Part:BBa_J23101:Design| More Information]''' |
| - | '''<partinfo>J23106</partinfo> | + | '''<partinfo>J23106</partinfo> Constitutive promoter family member (medium expression)''' <br> pMed is a medium consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. <partinfo>pSB3K3</partinfo>, <partinfo>BBa_J61002</partinfo> and <partinfo>pSB1AC3</partinfo> <br> '''[http://partsregistry.org/Part:BBa_J23106:Design| More Information]''' |
| - | '''<partinfo>J23109</partinfo> | + | '''<partinfo>J23109</partinfo> Constitutive promoter family member (low expression)''' <br> pLow is a Low consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. <partinfo>pSB3K3</partinfo>, <partinfo>BBa_J61002</partinfo> and <partinfo>pSB1AC3</partinfo><br> '''[http://partsregistry.org/Part:BBa_J23109:Design| More Information]''' |
| - | '''<partinfo> | + | '''<partinfo>R0010</partinfo> Promoter (lacI regulated)''' <br> This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein. <br> '''[http://partsregistry.org/Part:BBa_R0010:Design| More information]''' |
===Vectors=== | ===Vectors=== | ||
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'''<partinfo>pSB1A2</partinfo> pSB1A2 (Replaced by pSB1A3 in registry) ''' <br> pSB1A2 is a high copy number plasmid carrying ampicillin resistance. Together with pSB1AC3 the vector was used for most assemblies of our team, because the high copy number made it easy to work with and easy isolation. <br>'''[http://partsregistry.org/Part:pSB1A2:Design| More Information]''' | '''<partinfo>pSB1A2</partinfo> pSB1A2 (Replaced by pSB1A3 in registry) ''' <br> pSB1A2 is a high copy number plasmid carrying ampicillin resistance. Together with pSB1AC3 the vector was used for most assemblies of our team, because the high copy number made it easy to work with and easy isolation. <br>'''[http://partsregistry.org/Part:pSB1A2:Design| More Information]''' | ||
| - | '''<partinfo> | + | '''<partinfo>pSB2K3</partinfo> A low copy number base vector''' <br> A low copy number base vector which as a resistance against Kanamycin <br>'''[http://partsregistry.org/Part:pSB2K3:Design| More Information]''' |
| - | '''<partinfo>BBa_J61002</partinfo> A normal base vector''' <br> A normal base vector which has a rsistance against Ampicillin <br>'''[ | + | '''<partinfo>pSB3K3</partinfo> A low copy number base vector''' <br> A low copy number base vector which as a resistance against Kanamycin <br>'''[http://partsregistry.org/Part:pSB3K3:Design| More Information]''' |
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| + | '''<partinfo>BBa_J61002</partinfo> A normal base vector''' <br> A normal base vector which has a rsistance against Ampicillin <br>'''[http://partsregistry.org/Part:J61002:Design| More Information]''' | ||
'''<partinfo>BBa_J61035</partinfo> Vector we get with GVP''' <br> It's the backbone of our GVP part is and it a resistance against Ampicillin/Gentamycin<br>'''[[Team:Groningen/Modelling/Used Parts/BBa_J61035|More information]]''' | '''<partinfo>BBa_J61035</partinfo> Vector we get with GVP''' <br> It's the backbone of our GVP part is and it a resistance against Ampicillin/Gentamycin<br>'''[[Team:Groningen/Modelling/Used Parts/BBa_J61035|More information]]''' | ||
Revision as of 19:26, 16 October 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Used Parts
TODOMaybe some general information of parts used from the registry.
- To get to the partsregistry site of a particular part one can click on the name of the part
- To see what our main findings where (no details or derivations) regarding a particular part one can click on more information
GVP Constructs
GVP Gas Vesicle Proteins
This parts creates gas vesicles inside the cell wich enables it to float. It consist of 6064 base pairs. It's backbone is
More information
Name Part Main title with discription
About 100 to 200 words chracterizing the Parts and our experience with it ... bla...
More information
Miscellaneous
RBS ((Elowitz 1999) -- defines RBS efficiency)
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iGEM Groningen 2009 |
The ligation of a part behind the RBS succeded, and was confirmed by gel (correct vector size) and sequencing with VF2. We used this part in combination with several genes for building our biobricks e.g. . |
Promotors
Constitutive promoter family member (high expression)
pHigh is a high consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. , and
[http://partsregistry.org/Part:BBa_J23100:Design| More Information]
Constitutive promoter family member (high expression, reference)
pHigh is a high consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. , and
[http://partsregistry.org/Part:BBa_J23101:Design| More Information]
Constitutive promoter family member (medium expression)
pMed is a medium consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. , and
[http://partsregistry.org/Part:BBa_J23106:Design| More Information]
Constitutive promoter family member (low expression)
pLow is a Low consituative promotor. It consists of about 35 base pairs. It has been placed in the following vectors. , and
[http://partsregistry.org/Part:BBa_J23109:Design| More Information]
Promoter (lacI regulated)
This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.
[http://partsregistry.org/Part:BBa_R0010:Design| More information]
Vectors
High copy BioBrick assembly plasmid
pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. Together with pSB1A2 the vector was used for most assemblies of our team, because the high copy number made it easy to work with and easy isolation.
[http://partsregistry.org/Part:pSB1AC3:Design| More Information]
pSB1A2 (Replaced by pSB1A3 in registry)
pSB1A2 is a high copy number plasmid carrying ampicillin resistance. Together with pSB1AC3 the vector was used for most assemblies of our team, because the high copy number made it easy to work with and easy isolation.
[http://partsregistry.org/Part:pSB1A2:Design| More Information]
A low copy number base vector
A low copy number base vector which as a resistance against Kanamycin
[http://partsregistry.org/Part:pSB2K3:Design| More Information]
A low copy number base vector
A low copy number base vector which as a resistance against Kanamycin
[http://partsregistry.org/Part:pSB3K3:Design| More Information]
A normal base vector
A normal base vector which has a rsistance against Ampicillin
[http://partsregistry.org/Part:J61002:Design| More Information]
It's the backbone of our GVP part is and it a resistance against Ampicillin/Gentamycin
More information
