Team:HKUST/AttranctantProduction

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<li><a href="https://2009.igem.org/Team:HKUST/View3">Overview</a></li>
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In the 20th century, a large number of organic molecules have been identified as attractant towards drosophila, and many are now being produced for industrial use. Such compounds include 2-phenylethanol, eugenol, methyleugenol, isoeugenol, methylisoeugenol, ethanol, acetic acid and glucose, etc. This broad category of attractant molecules exhibit different levels of attraction for the insect.
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  In this sub-project, we are trying to use the yeast endogenous metabolic enzyme to produce a volatile insect attractant. Attractant production would be achieved by induced over-expression of an aromatic amino-transferase, the endogenous enzyme that can catalyze a reaction to yield 2-phenylethanol. This pathway will be triggered by the downstream signal from the GPCR, when FUS1 promoter is activated.
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<li><a href="https://2009.igem.org/Team:HKUST/Back3">Background</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Back3">Background</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Group3">Experimental design</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/Group3">Experimental design</a></li>

Revision as of 11:16, 17 October 2009

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Welcome

In the 20th century, a large number of organic molecules have been identified as attractant towards drosophila, and many are now being produced for industrial use. Such compounds include 2-phenylethanol, eugenol, methyleugenol, isoeugenol, methylisoeugenol, ethanol, acetic acid and glucose, etc. This broad category of attractant molecules exhibit different levels of attraction for the insect. In this sub-project, we are trying to use the yeast endogenous metabolic enzyme to produce a volatile insect attractant. Attractant production would be achieved by induced over-expression of an aromatic amino-transferase, the endogenous enzyme that can catalyze a reaction to yield 2-phenylethanol. This pathway will be triggered by the downstream signal from the GPCR, when FUS1 promoter is activated.

  • Background
  • Experimental design
  • Parts design
  • Experimental result
  • Future work
  • Reference
  • HKUST