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Team:Wisconsin-Madison/Notebook Protocols
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Revision as of 20:12, 17 October 2009
Protocols
- Plasmid Preparation by Alkaline Lysis
- Plasmid Preparation by Boiling Lysis
- Restriction Digestion
- Transformation of Plasmids into Competant Cells
- Preparation of Competent Cells
- Cloning Protocols
- Polymerase Chain Reaction (PCR)
- Ligation
- Digestion - Cloning
- Inoculation of Plasmids in Liquid Media
- Inoculation of Cyanobacteria
- Loading Gel Protocol
- Plasmid Clean-up
- Gel Extraction from Agar
- Sequencing Reaction
- Colony Polymerase Chain Reaction (PCR)
- Genomic DNA Extraction
