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September/18 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1.Min prep<br> result of nano drop<br> sample conc. 45A 275 ng/uL B 319 46A 212 B 302 47A 146 B 181 <br> 2.digesti...) |
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↓<br> | ↓<br> | ||
37°C,O/N<br> | 37°C,O/N<br> | ||
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 11:10, 20 October 2009
1.Min prep
result of nano drop
sample conc.
45A 275 ng/uL
B 319
46A 212
B 302
47A 146
B 181
2.digestion
K204027
| Vector | Insert | ||
|---|---|---|---|
| 1-6I | 3 | 19 | 9 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 13 | dH2O | 7 |
| total | 20uL | total | 20uL |
K204028
| Vector | Insert | ||
|---|---|---|---|
| 2-18H | 3 | 2-16N | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 13 | dH2O | 8 |
| total | 20uL | total | 20uL |
| Vector | Insert | ||
|---|---|---|---|
| 2-18F | 2 | 1-19B | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 14 | dH2O | 11 |
| total | 20uL | total | 20uL |
| Vector | Insert | ||
|---|---|---|---|
| 1-2M | 5 | 1-14L | 9 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 4 |
| total | 20uL | total | 20uL |
| Vector | Insert | ||
|---|---|---|---|
| 1-14N | 10 | 1-8K | 2 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 6 | dH2O | 14 |
| total | 20uL | total | 20uL |
| Vector | Insert | ||
|---|---|---|---|
| 1-1D | 5 | 33 | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 10 |
| total | 20uL | total | 20uL |
K204051
| Vector | Insert | ||
|---|---|---|---|
| 1-6I | 10 | 49 | 6 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 6 | dH2O | 10 |
| total | 20uL | total | 20uL |
↓
37°C, 2hr
↓
gel electrophoresis
gel cut
4.colony check
No. no. of colony 29 6 38 1
5.digestion
K204045
| Vector | Insert | ||
|---|---|---|---|
| 31 | 12 | 1-8I | 12 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 4 | dH2O | 4 |
| total | 20uL | total | 20uL |
K204046
| Vector | Insert | ||
|---|---|---|---|
| 32 | 12 | 1-8K | 12 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 4 | dH2O | 10 |
| total | 20uL | total | 20uL |
↓
37°C,O/N
