Team:Illinois/Vectors

(Difference between revisions)

Revision as of 19:36, 22 June 2009

This page contains maps of the plasmids used in our project.

Note: The PZE12luc plasmid is the parental plasmid of the PJU-334 linear fragment.

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-== '''Gels'''==
-This is the first gel of the PCR that we did of the pJU-334 DNA sequence. The band shows up at ~1.9 kbp. We expected it to be at 3.1 kbp.
-[[Image:Illinois-_Gel_1_of_PCR_of_pJU-334.jpg]]
-This is the second gel of the PCR that we did of the pJU-334 DNA sequence. The band shows up at ~11 kbp --oops. Again, we expected a band at ~3.1 kbp.
-[[Image:Illinois-_Gel_2_of_PCR_of_pJU-334.jpg]]
-This is the third gel of the PCR that we did of the pJU-334 DNA sequence. Here we see two band. A faint one, (where we expected the band to show up) at 3.1 kbp. The second, more prominent band is at ~1.9 kpb again.
-[[Image:Illinois-_Gel_3_of_pJU-334.jpg]]
-This is the fourth gel of the PCR that we did of the pJU-334 DNA sequence. This time, we put in all 45 micro liters of the DNA. Again, we got fainter bands at 3.1 kbp and stronger ones at ~1.9. Then we purified the 3.1 kbp fragments of DNA. We then used this purified DNA for another PCR. Next, we intend to run a gel of this, isolate the 3.1 kbp fragment, and use this to proceed with our experiment.
-[[Image:Illinois-_Gel_4_of_pJU-334.jpg]]
-Update: We just decided that maybe they told us to use the wrong primer for the anti-sense (they had gotten rid of part of the plasmid). They gave us the primer PLlacoB and we were supposed to use PLlacoD.
-So the question becomes, how come we got DNA fragments that were about the right size? Maybe the primers similar?
 == '''Vectors''' ==