"
Team:Berkeley Wetlab/Recipes
From 2009.igem.org
(Difference between revisions)
Jennbrophy (Talk | contribs) |
|||
| Line 13: | Line 13: | ||
pH 7.4<br> | pH 7.4<br> | ||
bring volume to 100 ml<br> | bring volume to 100 ml<br> | ||
| + | |||
| + | ====Artificial Seawater==== | ||
| + | 500 ml ddH2O<br> | ||
| + | 12.2 g NaCl<br> | ||
| + | 2.6 g MgCl2<br> | ||
| + | .57 g CaCl2<br> | ||
| + | |||
| + | ====Tris Buffer + 2% SDS==== | ||
| + | 50 mM Tris<br> | ||
| + | 150 mM NaCl<br> | ||
| + | 2% SDS<br> | ||
| + | pH 7<br> | ||
| + | |||
'''*For our Assay Protocols Visit the Passengers Tab''' | '''*For our Assay Protocols Visit the Passengers Tab''' | ||
Revision as of 19:34, 18 October 2009
|
Contents |
10X TBST
31.4 g TRIS HCl
80g NaCl
10ml Tween-20
bring volume to 1000ml
pH 7.5
1X TBS
605 mg Tris
876 mg NaCl
80 ml H2O
pH 7.4
bring volume to 100 ml
Artificial Seawater
500 ml ddH2O
12.2 g NaCl
2.6 g MgCl2
.57 g CaCl2
Tris Buffer + 2% SDS
50 mM Tris
150 mM NaCl
2% SDS
pH 7
*For our Assay Protocols Visit the Passengers Tab
