Wisconsin-Madison/20 June 2009

From 2009.igem.org

(Difference between revisions)
(New page: '''Ex 15: Inducing various Modified (Triple) ''E.Coli'' Strain Cultures 3''' Used salt LB cannot continue. Re-innoculate with salt-free LB Made liquid Cultures of E.Coli Strains in sal...)
 
Line 1: Line 1:
 +
== '''Calendar''' ==
 +
 +
<table>
 +
<html>
 +
<style type="text/css">
 +
table.calendar          { margin: 0; padding: 4px; }
 +
table.calendar td      { margin: 0; padding: 4px; vertical-align: top; }
 +
table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:120%; font-weight:bold; }
 +
table.month .dow td    { color:#00cc00; text-align:center; font-size:90%; }
 +
table.month td.today    { background-color:#ccff00; }
 +
table.month td {
 +
    border: none;
 +
    margin: 0;
 +
    padding: 0pt 0.5pt;
 +
    font-weight: bold;
 +
    font-size: 8pt;
 +
    text-align: right;
 +
    background-color: white;
 +
    }
 +
#bodyContent table.month a { background:none; padding:0 }
 +
.day-active { color:#0099ee }
 +
.day-empty  { color:#000000 }
 +
 +
</style>
 +
</html>
 +
 +
 +
{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
 +
</table>
 +
 +
{| align="center"
 +
|-valign="top"
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=04}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=05}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=06}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=07}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=08}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=09}}
 +
|align="center" width="150pt"|{{#calendar: title=Wisconsin-Madison |year=2009 | month=10}}
 +
|}
 +
 +
----
 +
 +
===='''June 20, 2009'''====
 +
'''Ex 15: Inducing various Modified (Triple) ''E.Coli'' Strain Cultures 3'''
'''Ex 15: Inducing various Modified (Triple) ''E.Coli'' Strain Cultures 3'''

Latest revision as of 02:57, 19 October 2009

Calendar

April
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13] [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17] [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23] [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27] [http://2009.igem.org/Wisconsin-Madison/28_April_2009 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]
May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5] [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12] [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16] [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
[http://2009.igem.org/Wisconsin-Madison/18_May_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
[http://2009.igem.org/Wisconsin-Madison/25_May_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Wisconsin-Madison/1_June_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
[http://2009.igem.org/Wisconsin-Madison/8_June_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
[http://2009.igem.org/Wisconsin-Madison/15_June_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_June_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
[http://2009.igem.org/Wisconsin-Madison/22_June_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_June_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
[http://2009.igem.org/Wisconsin-Madison/29_June_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
[http://2009.igem.org/Wisconsin-Madison/6_July_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
[http://2009.igem.org/Wisconsin-Madison/13_July_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
[http://2009.igem.org/Wisconsin-Madison/20_July_2009 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
[http://2009.igem.org/Wisconsin-Madison/3_August_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
[http://2009.igem.org/Wisconsin-Madison/10_August_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
[http://2009.igem.org/Wisconsin-Madison/17_August_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
[http://2009.igem.org/Wisconsin-Madison/24_August_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
[http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
[http://2009.igem.org/Wisconsin-Madison/7_September_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11] [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
[http://2009.igem.org/Wisconsin-Madison/14_September_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18] [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21] [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
[http://2009.igem.org/Wisconsin-Madison/28_September_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2] [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
[http://2009.igem.org/Wisconsin-Madison/5_October_2009 5] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9] [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
[http://2009.igem.org/Wisconsin-Madison/12_October_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
[http://2009.igem.org/Wisconsin-Madison/19_October_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29] [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30] [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]

June 20, 2009

Ex 15: Inducing various Modified (Triple) E.Coli Strain Cultures 3

Used salt LB cannot continue. Re-innoculate with salt-free LB

Made liquid Cultures of E.Coli Strains in salt-free LB;

1. BL21 (DE3)

2. K-12 MG1655 AaraBAD

3. K-12 MG1655 (wildtype)

4. DH10B


Ex 16: Need to redo:

A. PCR clean up: Taq PCR Product - Promega Wizard Genomic DNA Purification Kit

B. Plan: Same sticky ends, ProU upstream of GFP:

Backbone: GFP: Cut with E and S – 32 mg/uL – 3720 bp

Insert: ProU: Cut with E and X – 120 mg/uL – 360 bp

100 x 5 x 360/ 3720 = 50


C. Digestion: 10uL rxn no water


D. Purification: according to Promega Wizard Genomic DNA Purification Kit


E. Ligation: 1 uL ligase

1 uL buffer

3 uL water

1 uL ProU

4 uL GFP - overnight

Retrieved from "http://2009.igem.org/Wisconsin-Madison/20_June_2009"