Team:Freiburg bioware/cloning1

From 2009.igem.org

(Difference between revisions)

Revision as of 11:01, 19 October 2009

FREiGEM

Index

*FokA_pMA
*Foki_pMA
*Dig_pMA
*FluA_pMA
*DsbA_pMA
*StrepTag_pMA
*HisTag_pMA

Parts

FokA_pMA

back to index

FokA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

Commented GenBank file

FokA_pMA.txt

Foki_pMA

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Foki in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

Commented GenBank file

Foki_pMA.txt

Dig_pMA

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Dig in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

AA sequence of ORF-1:

MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

Commented GenBank file

Dig_pMA.txt

FluA_pMA

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FluA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

AA sequence of ORF-1:

MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

Commented GenBank file

FluA_pMA.txt

DsbA_pMA

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DsbA in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

AA sequence of ORF-1:

MKKIWLALAGLVLAFSASATG

Commented GenBank file

DsbA_pMA.txt

StrepTag_pMA (iGEM team Freiburg 08)

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StrepTag in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

AA sequence of ORF-1:

MAGWSHPQFEKTG

Commented GenBank file

StrepTag_pMA.txt

HisTag_pMA (iGEM team Freiburg 08)

back to index

StrepTag in pMA Vector

link to part registry

nucleotid sequence of ORF-1:
GCCGGCCATCATCATCATCATCATACCGGT

AA sequence of ORF-1:

AGHHHHHHTG

Commented GenBank file

HisTag_pMA.txt

@@ Line 113: / Line 113: @@
 *<a href="#4">Dig_pMA
 </a><br>
-*<a href="#5">DsbA_pMA
+*<a href="#5">FluA_pMA
+</a><br>
+*<a href="#6">DsbA_pMA
+</a><br>
+*<a href="#7">StrepTag_pMA
+</a><br>
+*<a href="#8">HisTag_pMA
 </a><br>
@@ Line 319: / Line 325: @@
 <br>
-<a name="4"></a>
+<a name="5"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;FluA_pMA<span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 376: / Line 382: @@
-<a name="5"></a>
+<a name="6"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA_pMA<span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 410: / Line 416: @@
-<a name="5"></a>
+<a name="7"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;StrepTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">
@@ Line 446: / Line 452: @@
-<a name="5"></a>
+<a name="8"></a>
 <h3 class="art-PostHeaderIcon-wrapper"> &nbsp;HisTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3>
 <div class="art-PostContent">