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Team:UNIPV-Pavia/Methods Materials/Glycerol stock
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Revision as of 16:11, 28 June 2009
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Glycerol stocks
(estimated time: 15 min + freezing time)
Materials needed:
- Sample of overnight incubated bacteria
- 80% glycerol
- Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
- Leave at -20°C for one day
- Move to -80°C the day after
