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IBB Pune/8 October 2009
From 2009.igem.org
(Difference between revisions)
(New page: I] Assembly. Digestions:- ES XP Constitutive promoter LacO Promoter RBS mRFP YcdB RBS ...) |
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Digestions:- | Digestions:- | ||
| - | ES | + | ES Constitutive promoter Promoter RBS YcdB |
| - | Constitutive promoter | + | |
| - | Promoter RBS | + | |
| - | YcdB | + | XP LacO mRFP GFP RBS |
| - | + | ||
| - | BKBN A2 | + | BKBN A2 from fGFP |
ligations | ligations | ||
Revision as of 14:41, 19 October 2009
I] Assembly.
Digestions:- ES Constitutive promoter Promoter RBS YcdB
XP LacO mRFP GFP RBS
BKBN A2 from fGFP
ligations Constitutive promoter + LacO Promoter RBS + mRFP Constitutive promoter + RBS YcdB + GFP Promoter RBS + GFP
II] PCR of YcdB with M13f and M13r
III] LuxI (AHL) assay with FeCl3 and hydroxylamine. FAILED :{
IV]
Transformation of ligated samples
