Team:KULeuven/Project Results
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- | | [[Image:weakness.png|thumb|400px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]] | + | | [[Image:weakness.png|thumb|400px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260}} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]] |
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Revision as of 14:49, 19 October 2009
Project Results
Our Results can be roughly divided in two sections, the biobrick assembly from starting parts in order to create our (test)-devices and secondly the characterizations of a part.
- First and foremost we succesfully assembled the following biobricks:
Partnumber Description
Antisense key
Sam5 + rbs
virA
VirG
VirB
Blue light promotor
Ech+fcs
Blp + GFP
Blp + RFP
EF + terminator
EF+ promoter+ terminator
Sam5 +sam8
Sams + promoter
Sams + promoter + comt
Sams+ comt+ promoter en terminator
lock
Sam8 + RBS
Ech + rbs
Fcs + rbs
All these parts were sent to the registry.
The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor, furthermore the effect of promotor strenght on blue light irradiance was examined.