Team:LCG-UNAM-Mexico/Parts

From 2009.igem.org

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|Designed by:
|Designed by:
|Jesus Abraham Avelar Rivas, Willebaldo Garcia Muñoz
|Jesus Abraham Avelar Rivas, Willebaldo Garcia Muñoz
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|}
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<br>
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<html>
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<body>
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<table border="0" align="center" width="900"><tr><th><img border="0" scr="http://partsregistry.org/images/partbypart/icon_coding.png"></th><th><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K242001">BBa_K242001</a></th></tr></table>
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</body>
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</html>
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{|valign="top" border="0" width="900px" cellpadding="10"
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|-
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|Part name:
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|Phage P2 cox transcriptional activator
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|-
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|Description:
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|Cox (Control of excision) is a coliphage P2 key regulator in charge of activating P4 Pll promoter, which leads transcription of the P4 replication operon. Another function for cox, hence its name, is to repress the activation of the inmunity repressor, which participates in P2 late gene induction, necessary for complete assembly of P4 viral particles.
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|-
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|Length:
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|294 bp
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|-
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|Designed by:
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|Luis Fernando Montaño Gutierrez
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|}
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<br>
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<html>
 +
<body>
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<table border="0" align="center" width="900"><tr><th><img border="0" scr="http://partsregistry.org/images/partbypart/icon_coding.png"></th><th><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K242001">BBa_K242001</a></th></tr></table>
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</body>
 +
</html>
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{|valign="top" border="0" width="900px" cellpadding="10"
 +
|-
 +
|Part name:
 +
|Phage P2 cox transcriptional activator
 +
|-
 +
|Description:
 +
|Cox (Control of excision) is a coliphage P2 key regulator in charge of activating P4 Pll promoter, which leads transcription of the P4 replication operon. Another function for cox, hence its name, is to repress the activation of the inmunity repressor, which participates in P2 late gene induction, necessary for complete assembly of P4 viral particles.
 +
|-
 +
|Length:
 +
|294 bp
 +
|-
 +
|Designed by:
 +
|Luis Fernando Montaño Gutierrez
 +
|}
 +
<br>
 +
<html>
 +
<body>
 +
<table border="0" align="center" width="900"><tr><th><img border="0" scr="http://partsregistry.org/images/partbypart/icon_coding.png"></th><th><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K242002">BBa_K242002</a></th></tr></table>
 +
</body>
 +
</html>
 +
{|valign="top" border="0" width="900px" cellpadding="10"
 +
|-
 +
|Part name:
 +
|Late P2 transcription activator Ogr
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|-
 +
|Description:
 +
|The Ogr protein is a key transcriptional regulator in the P2 P4 phage system. On one hand, it is a P2 replication-dependent inducer of the formation of capsid and tail proteins. On the other hand, it activates P4 sid operon which activates delta gene, capable of interacting with the same activation region as ogr. Therefore, late gene transcription is enhanced and hijacked by P4. This part is important to willingly activate production of phage P4 and therefore mediate the abundant multiplication of a P4 biobrick transduction vector.
 +
|-
 +
|Length:
 +
|238 bp
 +
|-
 +
|Designed by:
 +
|Luis Fernando Montaño Gutierrez
 +
|}
 +
<br>
 +
<html>
 +
<body>
 +
<table border="0" align="center" width="900"><tr><th><img border="0" scr="http://partsregistry.org/images/partbypart/icon_coding.png"></th><th><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K242029">BBa_K242029</a></th></tr></table>
 +
</body>
 +
</html>
 +
{|valign="top" border="0" width="900px" cellpadding="10"
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|-
 +
|Part name:
 +
|Colicin E9 DNase domain
 +
|-
 +
|Description:
 +
|Colicin E9 is able to degrade DNA. This DNase domain extracted by us is enough to get this function. It is inhibited in the presence of its immunity protein 9. 
 +
|-
 +
|Length:
 +
|431 bp
 +
|-
 +
|Designed by:
 +
|Jesus Abraham Avelar Rivas
|}
|}

Revision as of 23:11, 19 October 2009

Parts submitted to the registry

Coding parts

BBa_K242000

Part name: Colicin E3 rRNAse domain
Description: Colicin E3 is a bacteriocin targeting 16S rRNA in the 30S subunit at a single and specific site near to the 3' terminus. We extracted the ribonuclease domain which is enough for degrading RNA. Inhibited when Im3 is present.
Length: 343 bp
Designed by: Jesus Abraham Avelar Rivas, Willebaldo Garcia Muñoz


BBa_K242001

Part name: Phage P2 cox transcriptional activator
Description: Cox (Control of excision) is a coliphage P2 key regulator in charge of activating P4 Pll promoter, which leads transcription of the P4 replication operon. Another function for cox, hence its name, is to repress the activation of the inmunity repressor, which participates in P2 late gene induction, necessary for complete assembly of P4 viral particles.
Length: 294 bp
Designed by: Luis Fernando Montaño Gutierrez


BBa_K242001

Part name: Phage P2 cox transcriptional activator
Description: Cox (Control of excision) is a coliphage P2 key regulator in charge of activating P4 Pll promoter, which leads transcription of the P4 replication operon. Another function for cox, hence its name, is to repress the activation of the inmunity repressor, which participates in P2 late gene induction, necessary for complete assembly of P4 viral particles.
Length: 294 bp
Designed by: Luis Fernando Montaño Gutierrez


BBa_K242002

Part name: Late P2 transcription activator Ogr
Description: The Ogr protein is a key transcriptional regulator in the P2 P4 phage system. On one hand, it is a P2 replication-dependent inducer of the formation of capsid and tail proteins. On the other hand, it activates P4 sid operon which activates delta gene, capable of interacting with the same activation region as ogr. Therefore, late gene transcription is enhanced and hijacked by P4. This part is important to willingly activate production of phage P4 and therefore mediate the abundant multiplication of a P4 biobrick transduction vector.
Length: 238 bp
Designed by: Luis Fernando Montaño Gutierrez


BBa_K242029

Part name: Colicin E9 DNase domain
Description: Colicin E9 is able to degrade DNA. This DNase domain extracted by us is enough to get this function. It is inhibited in the presence of its immunity protein 9.
Length: 431 bp
Designed by: Jesus Abraham Avelar Rivas

Promoters and regulatory parts

Primers

Locations of visitors to this page


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