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From 2009.igem.org
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'''ADH''' | '''ADH''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAA | |
| + | GATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGC | ||
| + | CACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGC | ||
| + | CATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTAC | ||
| + | GCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGC | ||
| + | AACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCT | ||
| + | GATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGC | ||
| + | GCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATT | ||
| + | AGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTG | ||
| + | CTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATC | ||
| + | GATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGC | ||
| + | GTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGC | ||
| + | ACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTG | ||
| + | AAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTT | ||
| + | TTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTAC | ||
| + | GAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC | ||
*Description of function | *Description of function | ||
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol. | ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol. | ||
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'''groESL''' | '''groESL''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGT | |
| + | CCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCA | ||
| + | CTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT | ||
*Description of function | *Description of function | ||
heat-shock-responsive operon | heat-shock-responsive operon | ||
| Line 64: | Line 81: | ||
'''Rbc''' | '''Rbc''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAAC | |
| + | AGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTG | ||
| + | GGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTA | ||
| + | TGGAGGACTGACCTAG | ||
*Description of function | *Description of function | ||
RuBisCO promoter | RuBisCO promoter | ||
*Acknowedgment of sources and references | *Acknowedgment of sources and references | ||
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria | Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria | ||
Revision as of 02:12, 20 October 2009
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Parts
PDC
- Primary nucleaic acid sequence
ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGT TTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTG GGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATG AGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACG CCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCT GCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAA ACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCT ACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAA ACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATC GATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTC GCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCC CATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGC AAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGG ATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCA CATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACC ACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTG CTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGA GGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAAC AACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTG CTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCT GCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTG AATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGG GCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTAC CACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATC GAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCA CCAACGCCAAACAGTAA
- Description of function
PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.
- Acknowedgment of sources and references
Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)
ADH
- Primary nucleaic acid sequence
ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAA GATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGC CACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGC CATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTAC GCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGC AACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCT GATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGC GCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATT AGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTG CTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATC GATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGC GTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGC ACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTG AAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTT TTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTAC GAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
- Description of function
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.
- Acknowedgment of sources and references
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
groESL
- Primary nucleaic acid sequence
CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGT CCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCA CTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
- Description of function
heat-shock-responsive operon
- Acknowedgment of sources and references
Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31
Rbc
- Primary nucleaic acid sequence
CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAAC AGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTG GGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTA TGGAGGACTGACCTAG
- Description of function
RuBisCO promoter
- Acknowedgment of sources and references
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria
