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Team:IIT Madras/Notebook/Sandbox
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== Intermediates planned through PCR protocol == | == Intermediates planned through PCR protocol == | ||
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| + | These parts are essentially same as the those that occur in the construction strategies that are shown above. | ||
== Other new parts that are needed for control experiments == | == Other new parts that are needed for control experiments == | ||
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Revision as of 09:28, 20 October 2009
Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Plasmid 1
Plasmid 2
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
Plasmid 1
We had more than one way to build plasmid 1
Path 1
Path 2
Plasmid 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
Intermediates planned through PCR protocol
These parts are essentially same as the those that occur in the construction strategies that are shown above.
Other new parts that are needed for control experiments
