Team:HKUST/Result3

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<p>Constructs </p>
<p>Constructs </p>
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   We have successfully cloned the FUS1t gene into the multiple cloning site of the plasmid pRS426. The agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1 is shown in Figure 6. The other parts, the FUS1 promoter and the EGFP gene are still under construction.  Also, the ARO9 gene has been amplified from yeast genome.</p>
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   We have successfully cloned the FUS1t gene into the multiple cloning site of the vector pRS426. Agarose gel electrophoresis shows correct sizes after enzyme digestion of pRS426-tFUS1. The other parts, the FUS1 promoter and the EGFP gene are still under construction.  Also, the ARO9 gene has been amplified from the yeast genome.</p>
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Figure 6 agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1
 
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Latest revision as of 19:06, 21 October 2009

Salt and Soap template

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Constructs

We have successfully cloned the FUS1t gene into the multiple cloning site of the vector pRS426. Agarose gel electrophoresis shows correct sizes after enzyme digestion of pRS426-tFUS1. The other parts, the FUS1 promoter and the EGFP gene are still under construction. Also, the ARO9 gene has been amplified from the yeast genome.



  • Background
  • Experimental Design
  • Parts Design
  • Experimental Results
  • Future Work
  • References
  • HKUST