Team:Illinois/Research

From 2009.igem.org

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== '''Small RNAs''' ==
== '''Small RNAs''' ==
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*[http://www.ncbi.nlm.nih.gov/pubmed/15466019?dopt=Abstract: MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli]: Chen S, Zhang A, Blyn LB, Storz G J Bacteriol. 2004 Oct ; 186(20): 6689-97
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Concerns the method regulation of OmpC by the sRNA MicC
*[http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.58.030603.123841?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov The Small RNA Regulators of E. coli: Roles and Mechanisms]: Susan Gottesman, 2004
*[http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.58.030603.123841?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov The Small RNA Regulators of E. coli: Roles and Mechanisms]: Susan Gottesman, 2004

Revision as of 17:05, 30 June 2009

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Contents

Research

This page lists various sources used in our research organized by topic. Please note that most of the links to articles on this page will take you only to the abstract of the paper.

General

  • [http://www.neb.com/nebecomm/default.asp New England Biolabs Web Page]

Includes catalog for restriction endonucleases/other lab products, a resource called [http://tools.neb.com/NEBcutter2/index.php NEBcutter] that tells you what restriction endonucleases will cut your gene/DNA sequence of interest, and a tool called the [http://www.neb.com/nebecomm/DoubleDigestCalculator.asp? Double Digest Finder] that will tell you how compatible two restriction endonucleases are by comparing what conditions they work best in.

  • [http://genolist.pasteur.fr/Colibri/ Colibri]

Contains a complete list of genes in E. coli and their base pair sequences.

  • [http://www.ncbi.nlm.nih.gov/pubmed/ Pubmed]

A very useful online database for finding research papers and gene sequences.

Small RNAs

  • [http://www.ncbi.nlm.nih.gov/pubmed/15466019?dopt=Abstract: MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli]: Chen S, Zhang A, Blyn LB, Storz G J Bacteriol. 2004 Oct ; 186(20): 6689-97

Concerns the method regulation of OmpC by the sRNA MicC

  • [http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.58.030603.123841?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov The Small RNA Regulators of E. coli: Roles and Mechanisms]: Susan Gottesman, 2004

An overview on small RNAs and their functions, discussing the roles of Hfq and RNase E, and mentioning advantages to sRNA use.

  • [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4VNHRSC-B&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=997a0feec59ee5290a51b6ffcde5b7bb Regulatory RNAs in Bacteria]: Lauren S. Waters and Gisela Storz, Cell, Vol. 136, Issue 4, pg. 615-628, 2009

Discusses various RNA regulators in bacteria, including riboswitches and sRNAs. Gives examples of well-characterized sRNAs and lists advantages of sRNAs over other types of gene regulation.

  • [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1807950 Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo]: Johannes H. Urban and Jörg Vogel, 2007

Documents successful fusions of sRNA target sequences to GFP in order to study regulatory activity of various sRNAs.

  • [http://nar.oxfordjournals.org/cgi/content/abstract/33/3/1040 Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli]: Mitsuoki Kawano, April A. Reynolds, Juan Miranda-Rios, and Gisela Storz, 2005

Describes sRNAs that act on the 3' untranslated region (UTR) of a target mRNA.

  • [http://genesdev.cshlp.org/content/15/13/1637.long Identification of novel small RNAs using comparative genomics and microarrays]: Karen M. Wassarman, Francis Repoila, Carsten Rosenow, Gisela Storz, and Susan Gottesman, 2001

Describes sRNAs that act on the 3' untranslated region (UTR) of a target mRNA.

Modeling

  • [http://www.nature.com/msb/journal/v3/n1/full/msb4100181.html Regulation of Gene Expression by Small Non-Coding RNAs: A Quantitative View]: Yishai Shimoni, Gilgi Friedlander, Guy Hetzroni, Gali Niv, Shoshy Altuvia, Ofer Biham, and Hanah Margalit, 2007

Provides a basic model and differential equations for sRNA regulation of a gene, and quantitatively compares sRNA regulation with other types of regulation.

Protocols

  • [http://www.protocol-online.org/ Protocol Online]

Useful website for finding protocols online.

  • [http://www.5prime.com/media/29661/perfectprep%20spin%20mini%20kit%20manual_5prime_1048299_072007.pdf PerfectPrep Spin Mini Kit Manual]

Protocol for plasmid purification using the PerfectPrep Spin Mini Kit.

  • Supplementary Material: Translational Control and Target Recognition by Escherichia coli Small RNAs in Vivo: Johannes H. Urban and Jörg Vogel, 2007

Includes step-by-step protocols for fusion cloning into a standard plasmid (pXG-10), fusion cloning via 5' RACE (pXG-20), and operon fusion cloning into pXG-30.

  • [http://www.springerlink.com.proxy2.library.uiuc.edu/content/h752r3415261357p/ A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo]: Johannes H. Urban and Jörg Vogel, 2009

Details a modular two-plasmid system that uses GFP to study sRNA regulation in vivo.


Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.

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