Template:Imperial/09/Overview

(Difference between revisions)

Revision as of 12:51, 20 October 2009

Growth: Growth

We are growing the cells to a desired OD of 0.7 in order to ensure that there are enough cells to produce a wanted amount of proteins
Module 1: Protein Production: Module 1: Protein Production

The first module is induced with IPTG and triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest.
Module 2: Encapsulation: Module 2: Encapsulation

This second module is where the bacterium, after having produced the peptide of interest, produces colanic acid thus creating a protecting layer around itself to shelter it from the acidity of the stomach.
Module 3: Genome deletion: Module 3: Genome deletion

Module 3 occurs after encapsulation of the bacterium containing the produced peptide of interest. This module has the purpose of making the bacterium non viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The bacterium is thus unable to produce any proteins and therefore unable to survive.
Chemoinduction: Chemoinduction

Chemoinduction content
Autoinduction: Autoinduction

Autoinduction content
Thermoinduction: Thermoinduction

Thermoinduction content
Another: Time Regulation

Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.

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 	<dd id="g"><a href="https://2009.igem.org/Team:Imperial_College_London/M3" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
 		<span><div class="first">Module 3: Genome deletion</div>
-		<div class="rest" style="font-size:11px;">Module 3 occurs after encapsulation of the bacterium containing the produced peptide of interest. This module has the purpose of making the bacterium non viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The bacterium is thus unable to produce any proteins and therefore unable to survive. It leaves behind an encapsulated bag filled with our proteins of interest to then be delivered to the gut.</div></span>
+		<div class="rest">Module 3 occurs after encapsulation of the bacterium containing the produced peptide of interest. This module has the purpose of making the bacterium non viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The bacterium is thus unable to produce any proteins and therefore unable to survive.</div></span>
 	</a></dd>