Template:Imperial/09/Overview

(Difference between revisions)

Revision as of 12:51, 20 October 2009

Growth: Growth

We are growing the cells to a desired OD of 0.7 in order to ensure that there are enough cells to produce a wanted amount of proteins
Module 1: Protein Production: Module 1: Protein Production

The first module is induced with IPTG and triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest.
Module 2: Encapsulation: Module 2: Encapsulation

This second module is where the bacterium, after having produced the peptide of interest, produces colanic acid thus creating a protecting layer around itself to shelter it from the acidity of the stomach.
Module 3: Genome deletion: Module 3: Genome deletion

Module 3 occurs after encapsulation of the bacterium containing the produced peptide of interest. This module has the purpose of making the bacterium non viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The bacterium is thus unable to produce any proteins and therefore unable to survive.
Chemoinduction: Chemoinduction

Chemoinduction content
Autoinduction: Autoinduction

Autoinduction content
Thermoinduction: Thermoinduction

Thermoinduction content
Another: Time Regulation

Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.

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