Team:Freiburg bioware/cloning1

From 2009.igem.org

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<a name="12"></a>
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS418_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
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<div class="art-PostContent">
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
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<table border="5">
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<tr>
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<td align="center">
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<img src="https://static.igem.org/mediawiki/2009/thumb/8/84/Freiburg09_PJS418_dummy.jpg/600px-Freiburg09_PJS418_dummy.jpg" name="pJS418 Vector with strong SD sequence" width="480" height="480" />
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<br><p align="center">YFP(Venus) in pGA14 Vector</p>
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<li> <a href="http://partsregistry.org/Part:BBa_K243034">link to part registry</a>
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</td>
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<td align="left">
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nucleotid sequence of ORF-1:<br>
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ACCCGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGA
 +
GAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGC
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CGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAA
 +
AACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGC
 +
ACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCT
 +
GCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAG
 +
CGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAA
 +
TCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGC
 +
CATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCA
 +
GACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCA
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TCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTC
 +
GGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGAT
 +
AGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCT
 +
GAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGC
 +
AATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGACATCTCGGTAGTGGGATA
 +
CGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTT
 +
TCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGT
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GAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAA
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TACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGT
 +
TTCCCGACTGGAAAGCGGGCAGTGAGCGGTACCCGATAAAAGCGGCTTCCTGACAGGAGG
 +
CCGTTTTGTTTTGCAGCCCACCTCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCA
 +
CCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAA
 +
CAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTTCTAGCAGAAGGAGATATA
 +
CATCTAGATGGCCGGCAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC
 +
ATTTTGCCTTCCTGTTTTTGCTGACGGTCACCCAGAAACGCTGGCGAAAGTAAAAGATCT
 +
GGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATATCAACAGCGGTAAGAT
 +
CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCT
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ATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA
 +
CTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGG
 +
CATGACAGTAGGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAA
 +
CTTACTTCTGACAACGATCGGAGGACCGAAGGGCCTAACCGCTTTTTTGCGTAACATGGG
 +
GGATCATACAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGA
 +
CGAGCGTGACACCACGACGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGG
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CGAACTACTTACTCCAGCTTCCCGGCAACAATTAATGGACTGGATGAAAGCGGATAAAGT
 +
TGCAGGACCACTTCTGCGCTCGGTCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG
 +
AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGTGCTGGGGCCAGATGGTAAGCCCTC
 +
CCGTATCGTAGTTATCTACATGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACA
 +
GATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACCGGTTAATACTAGTAGCGGCC
 +
GCTGCAGAAGCTTGACCTGTGAAGTGAAAAATGGCGCACATTGTGCGACATTTTTTTTGT
 +
CTGCCGTTTACCGCTACTGCGTCACGGATCCCCACGCGCCCTGTAGCGGCGCATTAAGCG
 +
CGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCG
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CTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTC
 +
TAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAA
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AACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC
 +
CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACAC
 +
TCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATT
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GGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGT
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TTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTT
 +
TCTAAATACATTCAAATATGTATCCGCTCATGTCGAGACGTTGGGTGAGGTTCCAACTTT
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CACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGA
 +
GCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAA
 +
TGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAG
 +
ACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTT
 +
TATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATG
 +
GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTC
 +
CATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAG
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TTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCT
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AAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGT
 +
TTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAA
 +
TATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTC
 +
TGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG
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CAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGTGCTACG
 +
CCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGAAAGCAAATTCGACCCGGT
 +
CGTCGGTTCAGGGCAGGGTCGTTAAATAGCCGCTTATGTCTATTGCTGGTTTACCGGTTT
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ATTGACTACCGGAAGCAGTGTGACCGTGTGCTTCTCAAATGCCTGAGGCCAGTTTGCTCA
 +
GGCTCTCCCCGTGGAGGTAATAATTGCTCGACATGACCAAAATCCCTTAACGTGAGTTTT
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CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
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TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT
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TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA
 +
TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG
 +
CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA
 +
AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGG
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GCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA
 +
GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACA
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GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAA
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ACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT
 +
TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAC
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GGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG
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</td>
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</tr>
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</table>
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<h4>Commented GenBank file</h4>
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<li> <a href="https://static.igem.org/mediawiki/2009/3/3a/Freiburg09_PJS418_dummy.txt">pJS418.txt</a></li>
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<br>
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<br>
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<br>
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Revision as of 17:15, 20 October 2009

FREiGEM

 Index



 Parts

 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



    •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



    •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



    •  FluA_pMA

    back to index

    FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

    Commented GenBank file

  • FluA_pMA.txt



    •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



    •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt



    •  HisTag_pMA (iGEM team Freiburg 08)

    back to index

    HisTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GCCGGCCATCATCATCATCATCATACCGGT

    AA sequence of ORF-1:

    AGHHHHHHTG

    Commented GenBank file

  • HisTag_pMA.txt



    •  shortlinker26_pMA

    back to index

    shortlinker26 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGTG

    Commented GenBank file

  • shortlinker26_pMA.txt



    •  Middellinker38_pMA

    back to index

    Middellinker38 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • Middellinker38_pMA.txt



    •  LongLinkerA50_pMA

    back to index

    LongLinkerA50 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • LongLinkerA50_pMA.txt



    •  GSAT_linker_pMA

    back to index

    GSAT_linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY

    Commented GenBank file

  • GSAT_linker_pMA.txt



    •  SEG_Linker_pMA

    back to index

    SEG_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY

    Commented GenBank file

  • SEG_Linker_pMA.txt



    •  Split_Linker_pMA (iGEM team Freiburg 08)

    back to index

    Split_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGT

    AA sequence of ORF-1:

    MAGRPACKIPNDLKQKVMNHTG

    Commented GenBank file

  • Split_Linker_pMA.txt



    •  YFP(Venus)_pGA14 (iGEM team Freiburg 08)

    back to index

    YFP(Venus) in pGA14 Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC ATGGCATGGATGAACTGTATAAAACCGGTTAA

    AA sequence of ORF-1:

    MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY KTG

    Commented GenBank file

  • YFP(Venus)_pGA14.txt



    •  pJS418_Phagemid-dummy

    back to index

    YFP(Venus) in pGA14 Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ACCCGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGA GAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGC CGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAA AACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGC ACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCT GCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAG CGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAA TCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGC CATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCA GACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCA TCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTC GGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGAT AGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCT GAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGC AATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGACATCTCGGTAGTGGGATA CGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTT TCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGT GAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAA TACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGT TTCCCGACTGGAAAGCGGGCAGTGAGCGGTACCCGATAAAAGCGGCTTCCTGACAGGAGG CCGTTTTGTTTTGCAGCCCACCTCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCA CCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAA CAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTTCTAGCAGAAGGAGATATA CATCTAGATGGCCGGCAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC ATTTTGCCTTCCTGTTTTTGCTGACGGTCACCCAGAAACGCTGGCGAAAGTAAAAGATCT GGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATATCAACAGCGGTAAGAT CCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCT ATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA CTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGG CATGACAGTAGGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAA CTTACTTCTGACAACGATCGGAGGACCGAAGGGCCTAACCGCTTTTTTGCGTAACATGGG GGATCATACAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGA CGAGCGTGACACCACGACGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGG CGAACTACTTACTCCAGCTTCCCGGCAACAATTAATGGACTGGATGAAAGCGGATAAAGT TGCAGGACCACTTCTGCGCTCGGTCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGTGCTGGGGCCAGATGGTAAGCCCTC CCGTATCGTAGTTATCTACATGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACA GATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGACCGGTTAATACTAGTAGCGGCC GCTGCAGAAGCTTGACCTGTGAAGTGAAAAATGGCGCACATTGTGCGACATTTTTTTTGT CTGCCGTTTACCGCTACTGCGTCACGGATCCCCACGCGCCCTGTAGCGGCGCATTAAGCG CGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCG CTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTC TAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAA AACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCC CTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACAC TCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATT GGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGT TTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTT TCTAAATACATTCAAATATGTATCCGCTCATGTCGAGACGTTGGGTGAGGTTCCAACTTT CACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGA GCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAA TGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAG ACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTT TATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATG GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTC CATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAG TTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCT AAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGT TTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAA TATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTC TGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG CAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGTGCTACG CCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGAAAGCAAATTCGACCCGGT CGTCGGTTCAGGGCAGGGTCGTTAAATAGCCGCTTATGTCTATTGCTGGTTTACCGGTTT ATTGACTACCGGAAGCAGTGTGACCGTGTGCTTCTCAAATGCCTGAGGCCAGTTTGCTCA GGCTCTCCCCGTGGAGGTAATAATTGCTCGACATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGG GCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA GATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACA GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAA ACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAC GGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

    Commented GenBank file

  • pJS418.txt





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    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning1"