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| - | ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
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| - | {{:Team:Kyoto/Pad_start_dates}}
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| - | ===Monday, September 10===
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| - | ====To Do on 24 July====
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| - | *PCR (HIV TAT LALAA peptide,11R LALLA peptide)
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| - | *Electrophoresis
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| - | *Restriction Enzyme Digestion
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| - | ====Results====
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| - | =====Electrophoresis=====
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| - | [[Image:Kyoto_e_090724_1.png|250px]]
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| - | * Very clear bands were found. (See the gel image in right!)
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| - | * The bands are listed below.
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| | | | |
| - | {| border="1" style="text-align:center;width:50%"
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| - | |+Parts and Plasmid length
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| - | !!!3-O-2!!3-A-4
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| - | |-
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| - | |Parts length / bp||2837||487
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| - | |-
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| - | |Plasmid length / bp||3266||3266
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| - | |}
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| - |
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| - | =====Digestion=====
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| - | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
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| - |
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| - | {| border="1" style="text-align:center;width:50%"
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| - | |+Sample list
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| - | !NO.!!Sample Name!!Enzyme
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| - | |-align="center"
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| - | |1||3-O-2||Eco R1, PST1
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| - | |-align="center"
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| - | |2||3-A-4||Eco R1, PST1
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| - | |-align="center"
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| - | |3||3-O-2||Eco R1
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| - | |-align="center"
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| - | |4||3-A-4||Eco R1
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| - | |}
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| - |
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| - |
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| - | {{:Team:Kyoto/Pad_between_dates}}
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| - |
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| - |
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| - | ===Tuseday, September 11===
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| - | ====To Do on 24 July====
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| - | *Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
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| - | *Transformation
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| - |
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| - | ====Results====
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| - | =====Ligation=====
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| - | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
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| - |
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| - | {| border="1" style="text-align:center;width:50%"
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| - | |+Sample list
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| - | !NO.!!Sample Name!!Enzyme
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| - | |-align="center"
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| - | |1||3-O-2||Eco R1, PST1
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| - | |-align="center"
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| - | |2||3-A-4||Eco R1, PST1
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| - | |-align="center"
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| - | |3||3-O-2||Eco R1
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| - | |-align="center"
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| - | |4||3-A-4||Eco R1
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| - | |}
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| - |
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| - | =====Electrophoresis=====
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| - | [[Image:Kyoto_e_090724_1.png|250px]]
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| - | * Very clear bands were found. (See the gel image in right!)
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| - | * The bands are listed below.
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| - |
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| - | {| border="1" style="text-align:center;width:50%"
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| - | |+Parts and Plasmid length
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| - | !!!3-O-2!!3-A-4
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| - | |-
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| - | |Parts length / bp||2837||487
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| - | |-
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| - | |Plasmid length / bp||3266||3266
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| - | |}
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| - | {{:Team:Kyoto/Pad_endof_dates}}
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| - | <html></div><!-- /#rightcontents --></html>
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