Team:Kyoto/CiC/Notebook/week2

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(New page: ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == {{:Team:Kyoto/Pad_start_dates}} ===Monday, September 10=== ====To Do on 24 July==== *PCR (HIV TAT LALAA peptide,11...)
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==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
 
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{{:Team:Kyoto/Pad_start_dates}}
 
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===Monday, September 10===
 
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====To Do on 24 July====
 
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*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
 
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*Electrophoresis
 
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*Restriction Enzyme Digestion
 
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====Results====
 
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=====Electrophoresis=====
 
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[[Image:Kyoto_e_090724_1.png|250px]]
 
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* Very clear bands were found. (See the gel image in right!)
 
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* The bands are listed below.
 
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{| border="1" style="text-align:center;width:50%"
 
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|+Parts and Plasmid length
 
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!!!3-O-2!!3-A-4
 
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|-
 
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|Parts length / bp||2837||487
 
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|-
 
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|Plasmid length / bp||3266||3266
 
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|}
 
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=====Digestion=====
 
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 
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{| border="1" style="text-align:center;width:50%"
 
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|+Sample list
 
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!NO.!!Sample Name!!Enzyme
 
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|-align="center"
 
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|1||3-O-2||Eco R1, PST1
 
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|-align="center"
 
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|2||3-A-4||Eco R1, PST1
 
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|-align="center"
 
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|3||3-O-2||Eco R1
 
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|-align="center"
 
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|4||3-A-4||Eco R1
 
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|}
 
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{{:Team:Kyoto/Pad_between_dates}}
 
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===Tuseday, September 11===
 
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====To Do on 24 July====
 
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*Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
 
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*Transformation
 
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====Results====
 
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=====Ligation=====
 
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 
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{| border="1" style="text-align:center;width:50%"
 
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|+Sample list
 
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!NO.!!Sample Name!!Enzyme
 
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|-align="center"
 
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|1||3-O-2||Eco R1, PST1
 
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|-align="center"
 
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|2||3-A-4||Eco R1, PST1
 
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|-align="center"
 
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|3||3-O-2||Eco R1
 
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|-align="center"
 
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|4||3-A-4||Eco R1
 
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|}
 
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=====Electrophoresis=====
 
-
[[Image:Kyoto_e_090724_1.png|250px]]
 
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* Very clear bands were found. (See the gel image in right!)
 
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* The bands are listed below.
 
-
 
-
{| border="1" style="text-align:center;width:50%"
 
-
|+Parts and Plasmid length
 
-
!!!3-O-2!!3-A-4
 
-
|-
 
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|Parts length / bp||2837||487
 
-
|-
 
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|Plasmid length / bp||3266||3266
 
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|}
 
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{{:Team:Kyoto/Pad_endof_dates}}
 
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<html></div><!-- /#rightcontents --></html>
 

Revision as of 18:45, 20 October 2009