Team:Kyoto/CiC/Notebook/week2

From 2009.igem.org

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==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
 +
{{:Team:Kyoto/Pad_start_dates}}
 +
===Monday, September 10===
 +
====To Do on 24 July====
 +
*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
 +
*Electrophoresis
 +
*Restriction Enzyme Digestion
 +
====Results====
 +
=====Electrophoresis=====
 +
[[Image:Kyoto_e_090724_1.png|250px]]
 +
* Very clear bands were found. (See the gel image in right!)
 +
* The bands are listed below.
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Parts and Plasmid length
 +
!!!3-O-2!!3-A-4
 +
|-
 +
|Parts length / bp||2837||487
 +
|-
 +
|Plasmid length / bp||3266||3266
 +
|}
 +
 +
=====Digestion=====
 +
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 +
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Sample list
 +
!NO.!!Sample Name!!Enzyme
 +
|-align="center"
 +
|1||3-O-2||Eco R1, PST1
 +
|-align="center"
 +
|2||3-A-4||Eco R1, PST1
 +
|-align="center"
 +
|3||3-O-2||Eco R1
 +
|-align="center"
 +
|4||3-A-4||Eco R1
 +
|}
 +
 +
 +
{{:Team:Kyoto/Pad_between_dates}}
 +
 +
 +
 +
===Tuseday, September 11===
 +
====To Do on 24 July====
 +
*Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
 +
*Transformation
 +
 +
====Results====
 +
=====Ligation=====
 +
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 +
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Sample list
 +
!NO.!!Sample Name!!Enzyme
 +
|-align="center"
 +
|1||3-O-2||Eco R1, PST1
 +
|-align="center"
 +
|2||3-A-4||Eco R1, PST1
 +
|-align="center"
 +
|3||3-O-2||Eco R1
 +
|-align="center"
 +
|4||3-A-4||Eco R1
 +
|}
 +
 +
=====Electrophoresis=====
 +
[[Image:Kyoto_e_090724_1.png|250px]]
 +
* Very clear bands were found. (See the gel image in right!)
 +
* The bands are listed below.
 +
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Parts and Plasmid length
 +
!!!3-O-2!!3-A-4
 +
|-
 +
|Parts length / bp||2837||487
 +
|-
 +
|Plasmid length / bp||3266||3266
 +
|}
 +
{{:Team:Kyoto/Pad_endof_dates}}
 +
<html></div><!-- /#rightcontents --></html>

Revision as of 18:48, 20 October 2009

Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 10

To Do on 24 July

  • PCR (HIV TAT LALAA peptide,11R LALLA peptide)
  • Electrophoresis
  • Restriction Enzyme Digestion

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266
Digestion
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1



Tuseday, September 11

To Do on 24 July

  • Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
  • Transformation

Results

Ligation
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1
Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/week2"