Team:Wash U/Calendar-Home/29 June 2009

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(Difference between revisions)
(New page: Recipe for 0.8% agarose gel: 1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water) 2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop ...)
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== Monday June 29, 2009 ==
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Recipe for 0.8% agarose gel:
Recipe for 0.8% agarose gel:
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3) Cool, then add 2.5 microliters EtBr (wear gloves!).  Swirl, and pour into gel tray.  Let sit until ready for electrophoresis.
3) Cool, then add 2.5 microliters EtBr (wear gloves!).  Swirl, and pour into gel tray.  Let sit until ready for electrophoresis.
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-Made LB plates
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-Carried out digestion of plasmids
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-Carried out gel electrophoresis of digested plasmids
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-Carried out transformations of Biobrick parts

Revision as of 19:57, 29 June 2009



Monday June 29, 2009

Recipe for 0.8% agarose gel:

1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water)

2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop at 10-second intervals to swirl flask.

3) Cool, then add 2.5 microliters EtBr (wear gloves!). Swirl, and pour into gel tray. Let sit until ready for electrophoresis.


-Made LB plates

-Carried out digestion of plasmids

-Carried out gel electrophoresis of digested plasmids

-Carried out transformations of Biobrick parts

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