Team:UC Davis

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Revision as of 13:33, 21 October 2009

Homepage_UCdavis

            It is estimated that about one in every 133 Americans suffer from an autoimmune disorder called Celiac Disease (14, 11). Celiac Disease is a condition where the small intestines of an affected individual cannot properly digest gliadin, a protein present in gluten. When consumed, gliadin induces immune system responses, whose side-effects are, but not limited to abdominal pain, chronic diarrhea and vomiting (14). Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This can be very difficult as great varieties of food contain gluten. This inspired us to imagine an alternative treatment based on a probiotic organism that can survive and take residence in the stomach, where it would secrete an enzyme that will degrade gliadin.
           Then the design elements of this organism should satisfy at least two main criteria. First, it should be able to survive only in the stomach; secondly, secrete an enzyme that will degrade gliadin. We envision that these parts would not only be useful for our project, but also to other future projects.
       We decided to focus our summer project on building the first of two key elements required for our system:
                1. Produce an inducible secretion system
                2. Construct a biological pH sensor limiting this secretion system to the stomach

UC Davis iGEM team is sponsored by:

We want to thank Marc Facciotti for his helpful advice.
Special thanks to Suzy Fenton,Wayne Fenton, Jeanne Joe-Fenton and Wayne Fenton Jr.
Also we thank Charles H. Halsted for reviewing our material regarding celiac disease.

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 (14, 11). Celiac Disease is a condition where the small intestines of
 an
-affected individual cannot properly digest gliadin, a protein present in gluten.
+affected individual cannot properly digest gliadin, a protein present
+in gluten.
 When
 consumed, gliadin induces immune system responses, whose
 side-effects are,
 but not limited to abdominal pain, chronic diarrhea and vomiting (14).
 Currently, the only accepted treatment for this disorder is adhering to
 a
@@ Line 102: / Line 100: @@
 should be able to survive only in the stomach; secondly, secrete an
 enzyme
-that will degrade gliadin<i>.</i> We envision that these parts would not
+that will degrade gliadin<i>.</i> We envision that these parts would
+not
 only be useful for our project, but also to other future projects.</span><span
 style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p></span><br>
@@ Line 108: / Line 107: @@
 style="font-weight: bold;">&nbsp;&nbsp;&nbsp;
 &nbsp; &nbsp;
-&nbsp;&nbsp; We
+&nbsp;&nbsp; We decided to focus our summer project on building the
- decided to focus our summer project on building the first of two key elements
+first of two key elements
 required for
 our system:<o:p></o:p><br>
@@ Line 122: / Line 121: @@
 biological pH sensor limiting this secretion system to
 the
-stomach</a><br>
+stomach</a></big></span><a
+href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
+style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
+style="text-decoration: underline;"></span></span></a><span
+style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big
+style="font-weight: bold;"><br>
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+&nbsp;</span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><span
+style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span
+style="text-decoration: underline;"></span></span></a><a
 href="https://2009.igem.org/Team:UC_Davis/Project_Summary"><img alt=""
 src="https://static.igem.org/mediawiki/2009/a/ac/UCDAVIS_summary.png"
-style="border: 0px solid ; width: 132px; height: 101px;"></a><br>
+style="border: 0px solid ; width: 63px; height: 50px;"></a><span
+style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<span style="text-decoration: underline;"><br>
 </span> </span><a href="https://2009.igem.org/Team:UC_Davis/ph_sensor1"><b
 style="color: rgb(0, 0, 0);"><span
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 style="width: 182px; height: 108px;" alt=""
 src="https://static.igem.org/mediawiki/2009/c/c6/UCDAVIS_genome_center.jpg"><br>
-<font size="-1">Special thanks to <span style="font-weight: bold;">Suzy
+<font size="-1">We want to thank <span style="font-weight: bold;">Marc</span>
+<span style="font-weight: bold;">Facciotti</span> for his helpful
+advice.<br>
+Special thanks to <span style="font-weight: bold;">Suzy
 Fenton</span>,<span style="font-weight: bold;">Wayne Fenton, Jeanne
 Joe-Fenton </span>and<span style="font-weight: bold;"> Wayne Fenton Jr.</span>