Team:IPN-UNAM-Mexico/Notebook/October

From 2009.igem.org

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__FORCETOC__
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==='''''05-October-2009'''''===
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We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.
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----
 +
----
 +
 +
==='''''06-October-2009'''''===
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 +
The digest L2-3(BBa_K266000) dosen´t work, we have to repeat it.
 +
Left 17 hours gigest.
 +
----
 +
----
 +
 +
==='''''07-October-2009'''''===
 +
 +
We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone.
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Left  17 hours ligation overnight.
 +
----
 +
----
 +
 +
==='''''08-October-2009'''''===
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 +
Transformation  by head shock, plate and left overnight in 37°C camera.
 +
----
 +
----
 +
 +
==='''''09-October-2009'''''===
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 +
The transformation was successful we get five colonies.
 +
----
 +
----
 +
 +
==='''''12-October-2009'''''===
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Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.
 +
----
 +
----
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 +
==='''''13-October-2009'''''===
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 +
We have culture and prepare midi prep, with this we complete.
 +
----
 +
----
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==='''''15-October-2009'''''===
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Biobricks sent to the registry and final ligation in progress
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----
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----
{{Template:IPN-UNAM-Mexico-footer}}
{{Template:IPN-UNAM-Mexico-footer}}

Revision as of 23:33, 21 October 2009


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Contents

05-October-2009

We going to digest L2-3 (BBa_K266000) as insert XBAI and PSTI 17 hours digest in 37°C camera.

06-October-2009

The digest L2-3(BBa_K266000) dosen´t work, we have to repeat it. Left 17 hours gigest.

07-October-2009

We run out the digest and it is successful. Next step is attach to (BBa_K266006) as backbone. Left 17 hours ligation overnight.

08-October-2009

Transformation by head shock, plate and left overnight in 37°C camera.

09-October-2009

The transformation was successful we get five colonies.

12-October-2009

Left two colonies in LB medium inoculums overnight to prepare midi tomorrow.

13-October-2009

We have culture and prepare midi prep, with this we complete.

15-October-2009

Biobricks sent to the registry and final ligation in progress

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