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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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====Biobrciks recovered: RBS and CFP digestion==== | ====Biobrciks recovered: RBS and CFP digestion==== | ||
| - | *<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion ( | + | *<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I and ''Pst''I of plasmids containing the RBS and the CFP reporter in order to connect this parts.</p> |
*<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p> | *<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p> | ||
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| + | [[image:20090815_Digestões-Lig 1.jpg|500px|center]] | ||
*<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.</p> | *<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.</p> | ||
Revision as of 05:57, 21 October 2009
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