Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

(Difference between revisions)
(→DNA precipitation with sodium acetate)
(→DNA precipitation with sodium acetate)
Line 49: Line 49:
*Elute with 5 µl ddH2O
*Elute with 5 µl ddH2O
<br><br><br><br><br><br><br><br>
<br><br><br><br><br><br><br><br>
-
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
+
<br></td><td align='center' valign='bottom' width='300px'>
<div align='center'>
<div align='center'>
<table border='1'>
<table border='1'>

Revision as of 11:55, 30 June 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

</table
  • We use it only if the miniprep did not give us a high yield of DNA
  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5 µl ddH2O










  • Sodium acetate
  • Absolute ethanol
  • Ethanol 70%
  • ddH2O
  • Previously cut plasmid




Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Protocols/Precipitation"