Team:Wash U/Protocol
From 2009.igem.org
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'''Transformation'''<br> | '''Transformation'''<br> | ||
- | 1. Start thawing the competent cells on crushed ice.<br> | + | :1. Start thawing the competent cells on crushed ice.<br> |
2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> | 2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> | ||
3. Incubate the cells on ice for 30 minutes.<br> | 3. Incubate the cells on ice for 30 minutes.<br> |
Revision as of 15:49, 30 June 2009