Team:UNICAMP-Brazil/Notebooks/August 15

(Difference between revisions)

Revision as of 06:19, 21 October 2009

Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion (Protocol 14) with SpeI/PstI and XbaI/PstI of plasmids containing the RBS and the CFP reporter respectively in order to connect this parts.

After the digestion we isolated the target bands from the agarose gel, according to the Protocol 7.

The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.

Raíssa

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 ====Biobrciks recovered: RBS and CFP digestion====
-*<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I and ''Pst''I of plasmids containing the RBS and the CFP reporter in order to connect this parts.</p>
+*<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantificate the miniprep products (from 11/Aug) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I/''Pst''I and ''Xba''I/''Pst''I of plasmids containing the RBS and the CFP reporter respectively in order to connect this parts.</p>
 *<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p>