|
|
| Line 2: |
Line 2: |
| | | | |
| | | | |
| - | ===Summary===
| |
| | ===Achievement=== | | ===Achievement=== |
| - | ===Why do we need to use Iron-oxidizing bacteria for terraforming of Mars? === | + | |
| - | Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters.
| + | ===Introduciton ~Why do we need to use Iron-oxidizing bacteria for terraforming of Mars?~ === |
| - | Iron-oxidizing bacteria is autotrophic bacteria.
| + | |
| - | Iron-oxidizing bacteria gets energy by oxidizing Fe(Ⅱ).
| + | |
| - | To grow on Mars, iron-oxidizing bacteria require only energy injection of us because of composition of the Martian surface, crust and atmosphere. (リンク)
| + | |
| - | We propose culturing iron-oxidizing bacteria on Mars and producing organic matters.
| + | |
| - | Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli).
| + | |
| | | | |
| | ===Material and method=== | | ===Material and method=== |
| - | ==Iron-oxidizing bacteria==
| |
| - | Iron-oxidizing bacterium is a kind of autotrophic bacteria.
| |
| - | Iron-oxidizing bacteria reduce NAD<sup>+</sup> to NADH by oxidizing Fe(Ⅱ).
| |
| - | In laboratory, iron-oxidizing bacteria are cultured in [[#Protocols|9K medium]] which contains Fe(Ⅱ) and no organic matters.
| |
| - |
| |
| - | ==Cultivation==
| |
| - | We cultured ''Acidithiobacillus ferrooxidans'' (iron-oxidizing bacteria).
| |
| - | The medium for ''A.ferrooxidans'' was blue before cultivation owing to Fe(Ⅱ).
| |
| - | The presence of ''A.ferrooxidans'' changed the medium into brown one after culturing for two days.
| |
| - | This change was observed owing to the production of Fe(OH)<sub>3</sub>.
| |
| - | We confirmed the growth by using microscope of 200 magnifications.
| |
| - |
| |
| - | Shinya Tahara (a student member of Tokyo_tech team) conducted experiment with cultivation of A''.ferrooxidans''.
| |
| | | | |
| - | ===Protocols=== | + | ===Result=== |
| - | ====Preparation of 9K medium (for Acidithiobacillus ferrooxidans)====
| + | |
| - | *Prepare following A&B solution.
| + | |
| - | {|
| + | |
| - | |-
| + | |
| - | |[[Image:Tokyo_Tech_A_Solution.gif|200px|thumb|A Solution]]
| + | |
| - | |[[Image:Tokyo_Tech_B_Solution.gif|200px|thumb|B Solution]]
| + | |
| - | |}
| + | |
| - | *Sterilize A solution by autoclaving.
| + | |
| - | *Sterilize B solution by using filter.
| + | |
| - | *Mix A&B solution before use.
| + | |
| - | *A solution can be stored for 2 weeks.
| + | |
| | | | |
| - | ====Culturing method==== | + | ====Discussion==== |
| - | Culture in 9K medium at 30℃ with vigorous shaking.
| + | |
| - | Use the microscope when confirm the growth.
| + | |
| | | | |
| - | ==reference== | + | ==Reference== |
| | MEDIA FOORMULATIONS | | MEDIA FOORMULATIONS |
| | http://wdcm.nig.ac.jp/catalogue/ncim/document/Ncim_media.pdf | | http://wdcm.nig.ac.jp/catalogue/ncim/document/Ncim_media.pdf |
MEDIA FOORMULATIONS
http://wdcm.nig.ac.jp/catalogue/ncim/document/Ncim_media.pdf
"
