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Revision as of 09:48, 21 October 2009 (view source) Shinya (Talk | contribs) (→Cultivation of Acidithiobacillus ferrooxidans) ← Older edit		Revision as of 09:49, 21 October 2009 (view source) Shinya (Talk | contribs) (→Cultivation of Acidithiobacillus ferrooxidans) Newer edit →
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	===Cultivation of A''cidithiobacillus ferrooxidans''===		===Cultivation of A''cidithiobacillus ferrooxidans''===
-	We cultured ''Acidithiobacillus ferrooxidans'' (iron-oxidizing bacteria).<br\>
-	The medium for ''A.ferrooxidans'' was blue before cultivation owing to Fe(Ⅱ).<br\>
-	The presence of ''A.ferrooxidans'' changed the medium into brown one after culturing for two days.<br\>
-	This change was observed owing to the production of Fe(OH)<sub>3</sub>.<br\>
-	We confirmed the growth by using microscope of 200 magnifications.<br\>
	==Result==		==Result==

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Revision as of 09:49, 21 October 2009

Main

Team

Terraforming

Experiments

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Tokyo_Tech Parts]

Safety

Contents 1 Achievement 2 Introduciton ~Why do we need to use Iron-oxidizing bacteria for terraforming of Mars?~ 3 Material and method 3.1 Iron-oxidizing bacteria 3.2 Preparation of 9K medium (for iron-oxidizing bacteria) 3.3 Cultivation of Acidithiobacillus ferrooxidans 4 Result 5 Discussion 6 Reference

Achievement

• We cultured iron-oxidizing bacteria (Acidithiobacillus ferrooidans).

Introduciton ~Why do we need to use Iron-oxidizing bacteria for terraforming of Mars?~

Heterotrophic bacteria cannot live in native Martian environment because there are no organic matters on Mars, however autotrophic bacteria can live without organic matters.
Iron-oxidizing bacteria is autotrophic bacteria.
Iron-oxidizing bacteria gets energy by oxidizing Fe(Ⅱ). To grow on Mars, iron-oxidizing bacteria require only energy injection of us because of composition of the Martian surface, crust and atmosphere. (リンク)
We propose culturing iron-oxidizing bacteria on Mars and producing organic matters.
Accumulation of organic matters provides habitable environment for heterotrophic bacteria (for instance, E.coli).

Material and method

Iron-oxidizing bacteria

Iron-oxidizing bacterium is a kind of autotrophic bacteria.
Iron-oxidizing bacteria reduce NAD⁺ to NADH by oxidizing Fe(Ⅱ).
Iron-oxidizing bacteria grow with dissolved CO₂ as carbon source, not organic matters.
In laboratory, iron-oxidizing bacteria are cultured in 9K medium which contains Fe(Ⅱ) and no organic matters.

Preparation of 9K medium (for iron-oxidizing bacteria)

We Prepared following A and B solution.

A Solution

B Solution

We sterilized A solution by autoclaving. We sterilizde B solution by using filter.
We Mixed A and B solution before use.

Cultivation of Acidithiobacillus ferrooxidans

Result

Discussion

Reference

MEDIA FOORMULATIONS http://wdcm.nig.ac.jp/catalogue/ncim/document/Ncim_media.pdf

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