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Team:BIOTEC Dresden/Methods Vesicles
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| - | + | [[Image:Silicon wafer.jpg|200px|thumb|right|Silicon wafer we used for microfluidics chamber preparation]] | |
Production of flow chambers: | Production of flow chambers: | ||
* mix PDMS and curing agent in 10:1 ratio | * mix PDMS and curing agent in 10:1 ratio | ||
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* seal irreversibly by heating on plate at 60ºC for 6 hours | * seal irreversibly by heating on plate at 60ºC for 6 hours | ||
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Revision as of 20:25, 21 October 2009
Vesicles - Methods
Setup of the microfluidic system
The microfluidic system consists of a flow chamber made of Polydimethylsiloxane (PDMS) and a pump system that controls the flow rates of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows containment and imaging. Two types of chambers have been used, differing in the geometry of the space where droplets were produced. One featured T-junction, and the other a V-junction (Fig1).
Fig1: Different geometries of the intersection between aqueous and oil phase in flow chambers
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Production of flow chambers:
- mix PDMS and curing agent in 10:1 ratio
- degas and pour on wafer with etched microstructures
- polymerize on heat plate at 150 ºC for 30 min
- add unpolymerized PDMS mixture to points on microstructure where microtube inlets are to be pierced
- polymerize on heat plate at 150 ºC for 30 minutes
- remove polymerized PDMS from wafer, cut to fit onto glass cover slide (24 x 60 mm), and use clean needles (0.8 mm) or laser cutter to pierce tube inlets
- ionize PDMS and glass slide in plasma chamber for 30 sec to make it reactive
- align PDMS on glass slide and seal
- seal irreversibly by heating on plate at 60ºC for 6 hours
