Team:UNC Chapel Hill/2 July 2009
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==Notes== | ==Notes== | ||
- | *Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel | + | *Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel. [http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV] |
*Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria: | *Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria: | ||
**[http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1 | **[http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1 |
Revision as of 01:25, 6 July 2009
Notes
- Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel. [http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV]
- Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria:
- [http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1
- [http://partsregistry.org/Part:BBa_C0012 LacI] - Plate 2
- [http://partsregistry.org/Part:BBa_R0011 LacI Promoter] - Plate 3
- pBluescript (positive control) - Plate 4