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Team:IIT Madras/Results
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===Fluorescent Imaging=== | ===Fluorescent Imaging=== | ||
| - | [[Image:brightfield.jpg]] | + | [[Image:brightfield.jpg|center]] |
| - | [[Image:RFP.jpg]] | + | [[Image:RFP.jpg|center]] |
| - | [[Image:CFP.jpg]] | + | [[Image:CFP.jpg|center]] |
<html> | <html> | ||
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| + | <p>The above panel of images show cells co-transformed with both constructs, namely expressing CFP with Ampicillin and RFP with Chloramphenicol.<br> | ||
| + | figure (a) shows cells exposed to Bright Field.<br> | ||
| + | figure (b) shows same cells exposed to Red filter.<br> | ||
| + | figure (c) shows same cells exposed to Cyan filter. </p><br> | ||
For updated results, keep checking <a href="http://openwetware.org/wiki/IGEM:Indian_Institue_of_Technology_Madras/2009/Notebook/PLASMID_-_Plasmid_Locking_Assembly_for_Sustaining_Multiple_Insert_DNA/2009/10/31">here.</a> | For updated results, keep checking <a href="http://openwetware.org/wiki/IGEM:Indian_Institue_of_Technology_Madras/2009/Notebook/PLASMID_-_Plasmid_Locking_Assembly_for_Sustaining_Multiple_Insert_DNA/2009/10/31">here.</a> | ||
Revision as of 18:14, 21 October 2009
Contents |
Results
Comparing the differences in the growth rates of cells with and without plasmids in various media
Growth curves
Fig 8.1: DH5a grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
Fig 8.2: RFP (pSB1C3) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
Fig 8.3: CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
Fig 8.4: RFP (pSB1C3) - CFP (pSB1A2) contanining cells grown in LB containing no antibiotic, LB containing Ampicillin, LB containing Chloramphenicol, LB containing both antibiotics.
Fig 8.5: All 4 strains in media without antibiotics.
Fig 8.6: All 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
Fig 8.7: log (OD600) vs Time plot for all 4 strains in media without antibiotics.
Fig 8.8: log (OD600) vs Time plot for all 4 strains in their corresponding antibiotic media - DH5a in Lb without antibiotics, RFP (pSB1C3) in LB with Chloramphenicol, CFP (pSB1A2) in LB with Ampicillin, RFP (pSB1C3) - CFP (pSB1A2) cells in LB with Chloramphenicol and Ampicillin.
Modeling
For updated results, keep checking here.
Fluorescent Imaging
The above panel of images show cells co-transformed with both constructs, namely expressing CFP with Ampicillin and RFP with Chloramphenicol.
figure (a) shows cells exposed to Bright Field.
figure (b) shows same cells exposed to Red filter.
figure (c) shows same cells exposed to Cyan filter.
For updated results, keep checking here.
